Proven Stem Cell Markers and Multiple Applications

More Bang for Your Buck!

Our Stem Cell Markers remain a growth engine for Neuromics. One of the reasons that you would want to check out offerings are that we have selected markers that can be used in multiple applications.

For example, many of our markers can be used for immunostaining assays to confirm your stem cells are expressing the correct proteins and then these calls can further characterized and purified by Flow Cytometry, Here's an example of  one of our Nestins being used for these two applications.

Figures: (A): Nestin is present in both cell lines, . (B) Corresponding validation by immunofluorescence analysis. (C) Quantitation of nestin intracellular antigen detection (n=3). Error bars indicate standard deviation. DOI: 10.1371/journal.pone.0068519 .

Here are Stem Cell Markers that are designed for Multiple Applications. If you can cover your required applications with one antibody, the net results is a big cost savings!

Name	Catalog #	Type	Species	Applications	Size	Price
5T4	GT15239	Sheep IgG	M	ICC; WB; FC; E	100 ug	$365
ACE/CD143	GT15145	Goat IgG	H; R	IHC; WB; FC; E	100 ug	$365
Activin RIA	GT41003	Goat IgG	H	IHC; WB; E	100 ug	$250
Activin RIIA	GT15149	Goat IgG	H	IHC; WB; E	100 ug	$365
BAX	RA30008	Rabbit IgG	H	IHC; WB	50 ug	$425
BDNF	CH15000	Chicken IgY	H; R	IHC; WB; E	100 ug	$365
BDNF	MO15115	Mouse IgG	H	IHC; WB; E	500 ug	$325
Bmi1	RA25083	Rabbit IgG	B; Ca; Ch; H; M; Pr; R; Rb; Ze	ICC; IF; WB	100 ul	$265
BMP-2/4	GT15053	Goat IgG	H; R	ICC; IHC; WB; E	100 ug	$365
BMP-3b/GDF-10	GT15166	Goat IgG	H	IHC; WB; E	100 ug	$365
BMP-4	MO20038	Mouse IgG	H	IHC; WB	100 ul	$195
BMP-5	GT15054	Goat IgG	H; R	ICC; IHC; WB; E	100 ug	$365
BMP-6	GT15167	Goat IgG	H	IHC; WB; E	100 ug	$365
BMP-8	GT15161	Goat IgG	H; R	IHC; WB; FC; IP; E	100 ug	$365
BOC	GT15229	Goat IgG	M; R	IHC; WB; E	100 ug	$365
Brachyury	GT15224	Goat IgG	H; M	IHC; WB; E	100 ug	$365
Brg1	GT15244	Goat IgG	H; M	ICC; WB	100 ug	$365
c-Myc	RA25085	Rabbit IgG	H	IF; WB	100 ul	$355
phospho-CaMKII (Thr286)	RA18007	Rabbit IgG	H; M; Pr; R	IHC; WB	100 ul	$370
CD11b/c (OX-42)	RA25012	Rabbit IgG	H; M	IHC; WB; FC	100 ul	$285
CD11b (OX-42)	CH23021	Chicken IgY	H; M	IHC; WB	100 ul	$99
CCR1/CD191	MO15088	Mouse IgG	H	IHC; FC	100 ug	$325
CD31/PECAM-1	GT15190	Sheep IgG	H	IHC; WB; E	100 ug	$365
CD43 (Clone MT1)	MO20006	Mouse IgG	H	IHC; WB	100 ul	$150
CD44 Variant 3	MO20007	Mouse IgG	H	IHC; WB	100 ul	$150
CCR5	MO15077	Mouse IgG	H	IHC; FC	100 ug	$325
CCR6	MO15087	Mouse IgG	H	FC; IHC	100 ug	$255
CDCP1/CD138	GT15233	Goat IgG	H	IHC; WB; FC; E	100 ug	$365
CDO	GT15162	Goat IgG	M; R	IHC; WB; E	100 ug	$365
CXCL8/IL-8	GT15156	Goat IgG	H	IHC; WB; E	100 ug	$365
CXCR1/IL-8 RA	MO15072	Mouse IgG	H	IHC; FC	500 ug 100 ug	$325 $89
CXCR2/IL-8 RB	MO15073	Mouse IgG	H	IHC; FC; NB	100 ug	$325
CXCR4	MO15022	Mouse IgG	H	FC; IHC	100 ug	$325
Chordin	GT15191	Goat IgG	H; R	IHC; WB; E	100 ug	$365
Cripto1	RA25091	Rabbit IgG	H; M	FC; ICC; IF; WB	100 ul	$295
Dkk-1	GT30000	Goat IgG	H	IHC; WB; E	50 ug	$365
Dkk-2	GT15222	Goat IgG	M	IHC; WB; E	100 ug	$365
Desert Hedgehog	GT15067	Goat IgG	M	IHC; WB; E	100 ug	$365
Doublecortin/DCX	MO22113	Mouse IgG	B; H; M; P; R	IF; WB	100 ul	$295
Endoglin/CD105	GT15247	Goat IgG	H	FC; ICC; IHC; WB	100 ug	$365
Endoglin/CD105	MO15124	Rat IgG	M	FC; ICC; IHC; WB	100 ug	$265
EphA4	GT15035	Goat IgG	M; R	IHC; WB; E	100 ug	$365
Ephrin-A2	GT15025	Goat IgG	M; R	ICC; WB; E	100 ug	$365
Ephrin-B2	GT15026	Goat IgG	M; R	IHC; WB; FC; E	100 ug	$365
FGF-2	MO27001	Mouse IgM	H	WB; IP; E	500 ug	$175
FGF R5 beta	GT15093	Goat IgG	M; R	ICC; IHC; WB; E	100 ug	$365
FGF-9	GT15228	Goat IgG	H	IHC; WB; E	100 ug	$365
Frizzled 4/CD344	GT15217	Goat IgG	M	IHC; WB; E	100 ug	$365
gp130/CD130	MO15024	Rat IgG	M	IHC; WB; FC	100 ug	$255
GATA-1	MO15035	Mouse IgG	H	IHC; WB	100 ug	$255
GATA-2	GT15099	Goat IgG	H	ICC; WB	100 ug	$365
GDF-3	GT15176	Goat IgG	M	IHC; WB; E	100 ug	$365
GDF-5	GT15177	Goat IgG	M	IHC; WB; E	100 ug	$365
GFAP	CH23011	Chicken IgY	H; M	ICC; IF; IHC; WB	200 ul	$265
GFAP	MO15052	Mouse IgG	H; R	IHC; WB; E	100 ul	$255
GFAP	RA22101	Rabbit IgG	Ca; H; M; R	ICC; IHC; WB	100 ul	$275
GFAP	CH22102	Chicken IgY	H; M; Pr; R	ICC; IHC; WB	100 ul	$275
GPR49	RA25071	Rabbit IgG	H	FC; ICC; IF; IHC	100 ul	$315
HNF-3beta/FoxA2	GT15186	Goat IgG	H	IHC; WB; E	100 ug	$365
Islet-1	GT15051	Goat IgG	H; M	ICC; WB	100 ug	$365
Jagged1	GT15171	Goat IgG	H	E; IHC; WB	100 ug	$365
JARID2	RA25095	Rabbit IgG	H; M	ICC; IF; WB; IP	100 ul	$245
Ki67	RA25039	Rabbit IgG	H; M; P	ICC; IF; IHC	500 ul	$350
c-Kit/CD117	RA14132	Rabbit IgG	H; M; R	IF; IHC; FC	100 ul	$350
MAP2 (Microtubule assoc. protein 2)	CH22103	Chicken IgY	H; M; Pr; R	ICC; IHC; WB	50 ul	$295
MOG	GT15141	Goat IgG	H	IHC; WB; E	100 ug	$365
Mash1	GT15216	Goat IgG	M	IHC; WB; E	100 ug	$365
Mash1	MO15048	Rat IgG	H; M	ICC; WB; E	100 ug	$255
Musashi-1	MO15049	Mouse IgG	H	ICC; IHC; WB; E	100 ug	$255
Musashi-1	GT15116	Goat IgG	H	ICC; IHC; WB; E	100 ug	$365
NCAM-L1/CD171	GT15143	Goat IgG	H	IHC; WB; E	100 ug	$365
Nestin	MO15012	Mouse IgG	H; Pr	FC; ICC; IHC	100 ug	$265
Nestin	MO15056	Mouse IgG	M; R	FC; ICC; IHC	100 ug	$255
Nestin	CH23001	Chicken IgY	H; M	ICC; IHC; WB	100 ul	$99
Nestin	GT15114	Goat IgG	M; R	IHC; WB; E	100 ug	$365
Netrin-1	CH23002	Chicken IgY	H; M	ICC; IHC; WB	100 ul	$99
Netrin-4	GT15164	Goat IgG	H	IHC; WB; E	100 ug	$365
NeuroD1	GT15231	Goat IgG	H; M	ICC; WB	100 ug	$365
Neurofilament NF-H	CH22104	Chicken IgY	H; M; Pr; R	ICC; IHC; WB	50 ul	$275
Neurofilament NF-H, phosphylated	MO22103	Mouse IgG	Ch; H; M; Pr; R	ICC; IHC; WB	100 ul	$225
Neurofilament NF-L	CH22105	Chicken IgY	Ca; Ch; H; M; R	ICC; IHC; WB	100 ul	$275
Neurofilament NF-L	MO22104	Mouse IgG	Ca; Ch; H; M; R	ICC; IHC; WB; E	100 ul	$275
Neurofilament NF-M	CH22106	Chicken IgY	Ch; H; M; R	ICC; IHC; WB	100 ul	$275
Neurofilament NF-M	MO22105	Mouse IgG	Ch; H; M; R	ICC; IHC; WB	100 ul	$225
Neurofilament alpha-internexin/NF66	CH22101	Chicken IgY	H; M; R	FC; IF	100 ul	$275
Noggin	GT15159	Goat IgG	M	ICC; IHC; WB; E	100 ug	$365
Notch2	GT15124	Goat IgG	R	IHC; WB; FC; E	100 ug	$365
Notch3	RA19070	Rabbit IgG	H; R	IHC; WB	100 ug	$215
Nucleostemin	GT15050	Goat IgG	H; M; R	ICC; WB; E	100 ug	$365
Nurr1	RA19067	Rabbit IgG	M; R	IHC; WB	100 ul	$275
Oct3/4	GT15052	Goat IgG	H; M	ICC; WB	100 ug	$365
Olig2	GT15132	Goat IgG	H; M	IHC; WB; E	100 ug	$365
Olig2	RA25081	Rabbit IgG	H; M; R	ICC; IP; IHC; WB	100 ul	$395
Oligodendrocyte Marker O1	MO15001	Mouse IgM	H; M; R	IHC; FC	50 ug	$215
Oligodendrocyte Marker O4	MO15002	Mouse IgM	H; M; R	FC; IHC	50 ug	$215
Otx2	GT15095	Goat IgG	H; M	ICC; WB; E	100 ug	$365
Otx2	MO15039	Mouse IgG	H	ICC; IHC; WB	100 ug	$255
PDX1/IPF1	GT15187	Goat IgG	H	IHC; WB; E	100 ug	$365
PLP (Proteolipid Protein)	CH23008	Chicken IgY	H; M	ICC; IHC; WB	100 ul	$99
Patched homolog 1 (PTCH1)	MO15102	Rat IgG	M; R	IF; IHC; WB; E	100 ug	$255
Patched homolog 2 (PTCH2)	GT15215	Rabbit IgG	H; R	IHC; WB; FC; E	100 ug	$365
Prominin 2	MO15051-100	Mouse IgG	H	ICC; FC	100 ug	$255
Ret	GT15002	Goat IgG	M; R	IHC; WB; E	100 ug	$365
Ret Biotinylated	GT15002B	Goat IgG	M; R	IHC; WB	50 ug	$395
RGM-A	GT15169	Sheep IgG	M	IHC; FC; E	100 ug	$365
RGM-B	GT15212	Sheep IgG	M	IHC; FC; E	100 ug	$365
RUNX2	GT15188	Goat IgG	H	E; ICC; WB	100 ug	$365
S100B	RA25022	Rabbit IgG	H; M; R	IHC; WB; IP	100 ug	$365
S100B	GT15160	Goat IgG	H	IHC; WB; E	100 ug	$365
SLAIN1	GT15232	Goat IgG	H; M	ICC; WB	100 ug	$365
SMAD1	GT15219	Goat IgG	H; R	IHC; WB; E	100 ug	$365
SMAD3	RA30033	Rabbit IgG	B; Ch; H; M; P; R	IHC; WB; E	50 ug	$365
SMAD5	GT15192	Goat IgG	H; M; R	IHC; WB; E	100 ug	$365
Snail	GT15245	Goat IgG	H	ICC; WB; IP	100 ug	$365
Sonic Hedgehog	GT15032	Goat IgG	M; R	ICC; WB; E	100 ug	$365
SOX1	GT15208	Goat IgG	H	IHC; WB; E	100 ug	$365
SOX2	MO15040	Mouse IgG	H	IHC; WB	100 ug	$255
SOX2	GT15098	Goat IgG	H	IHC; WB; E	100 ug.	$375
SOX2	RA25021	Rabbit IgG	H; M	ICC; IHC; WB	100 ul	$275
SOX3	GT15119	Goat IgG	H	ICC; WB; E	100 ug	$365
SOX6	RA19023	Rabbit IgG	H; M; Pr; R	IHC; WB	100 ug	$225
SOX9	GT15207	Goat IgG	H	IHC; WB; E	100 ug	$365
SOX10	GT15218	Goat IgG	H	IHC; WB; E	100 ug.	$365
SOX17	GT15094	Goat IgG	H	ICC; WB; E	100 ug	$365
SOX21	GT15209	Goat IgG	H; M	IHC; WB; E	100 ug	$365
SSEA3	RA25027	Rabbit IgG	H; M	ICC; WB; FC; IP	100 ul	$275
Stella/Dppa3	GT15240	Goat IgG	M	IHC; WB; E	100 ug	$365
Synapsin	RA18010	Rabbit IgG	M; R	IHC; WB	100 ul	$370
Synaptophysin	MO20000	Mouse IgG	H; R	IHC; WB	100 ul	$175
TCF4 (C48H11)	RA18032	Rabbit IgG	Ch; H; M; R	WB; IP	100 ul	$325
TCF4 (C9b9)	RA18033	Rabbit IgG	H; M; R	IF; WB; IP	100 ul	$325
TGF beta 1	MO15106	Mouse IgG	H; M; Pr; R	IHC; WB; FC; E	500 ug	$325
TGF beta RII (type2)	GT15073	Goat IgG	H	IHC; WB; E	100 ug	$345
TIE1	GT15147	Goat IgG	H	IHC; WB; E	100 ug	$365
TIE2/CD202b	GT15148	Goat IgG	H	IHC; WB; E	100 ug	$365
TP63/TP73L	GT15189	Goat IgG	H	ICC; WB; E	100 ug	$365
TRA-1-60	MO25035	Mouse IgM	H	ICC; IF; WB; FC	100 ul	$275
TRA-1-81	MO25034	Mouse IgM	H	IF; IP; IHC; WB	100 ul	$245
Tuj 1 (Neuron-specific class III beta-tubulin)	MO15013	Mouse IgG	H	ICC; IF; IHC; WB	100 ug	$265
Tuj 1 (Neuron-specific class III beta-tubulin)	CH23005	Chicken IgY	H; M	ICC; IHC; WB	100 ul	$99
Tyrosine Hydroxylase	MO20001	Mouse IgG	H; M; R	ICC; WB	100 ul	$200
Tyrosine Hydroxylase	MO22941	Mouse IgM	M; Pr; R	IHC; WB	100 ul	$350
Tyrosine Hydroxylase	CH23006	Chicken IgY	H; M	ICC; IHC; WB	100 ul	$99
Tyrosine Hydroxylase	SO25000	Sheep IgG	H; M; Pr; R	ICC; IF; IHC; WB	100 ul	$325
phospho-Tyrosine Hydroxylase (Ser40)	RA18026	Rabbit IgG	R	IF; IHC; WB; IP	100 ul	$335
Vimentin	CH23010	Chicken IgY	H; M	ICC; IHC; WB	100 ul	$99
Vimentin	CH22108	Chicken IgY	H; M; R	IHC; WB	100 ul	$275
ZIC3	GT15241	Sheep IgG	H; M	ICC; WB	100 ug	$365
ZIC1	GT15242	Goat IgG	H; M	ICC; IHC; WB	100 ug	$365

Should you ever have questions on any of our solutions, please contact me directly @ 612-801-1007 or pshuster@neuromics.com. Thank you. Pete Shuster, CEO & Owner, Neuromics

3-D Screens Predict Breast Cancer Metastasis

Cell-ECM Interactions

3-D culturing solutions and methods are increasingly being used to created more in vivo like assays.

Here researchers use our Human Collagen IV extracellular matrix protein (ECM) to develop a simple biomaterial platform with systematic control over the ECM protein density and composition to determine if integrin binding governs how metastatic cells differentiate between secondary tissue sites: L.E. Barney, E.C. Dandley, L.E. Jansen, N.G. Reich, A.M. Mercurio, and S.R. Peyton. A cell–ECM screening method to predict breast cancer metastasis. Integr Biol (Camb). 2015 Feb 10; 7(2): 198–212. doi: 10.1039/c4ib00218k.

This publication details the creation of an in vitro fingerprint that is predictive of in vivo metastasis.

Figure: Biomaterial platform for integrin-mediated phenotyping. (a) Breast cancer cell lines with their known in vivo metastatic tropisms.  (b) Three distinct ECM microenvironments regulate integrin binding. (c) Adhesion and motility phenotypes of the MDA-MB-231 cell line. Black: ECM 1; blue: ECM 2; green: ECM 3.

Figure: Correlations between adhesion and migration responses identify potent integrin antibodies in vitro. Pairwise comparisons between adhesion and migration measurements in the (a, b) MDA-MB-231 and (c, d) SkBr3 cell lines across normal, EGF-stimulated, and integrin antibody conditions. Arrows highlight conditions where integrin antibodies increased migration metrics. Spearman correlations are indicated on each plot with two-tailed p-values. Circle: ECM 1; square: ECM 2; triangle: ECM 3; black: normal; green: EGF; blue: anti-β1 integrin; red: anti-α2 integrin; orange: anti-α6 integrin. (e, f) SkBr3 migration mechanisms are displayed via 10 random cell paths under (e) normal and (f) anti-β1 conditions. Red paths identify cells detaching and adhering elsewhere on the surface. Inset: representative images of cell morphology. Scale bar is 25 μm. (g) Individual cells that invaded into an overlaid 3D collagen gel from the ECM 1 surface after 48 hours. Bar indicates mean distance invaded of all invading cells. Inset: schematic of cells invading upward from the ECM surface into an overlaid gel.

A richer understanding of cancer metastasis is important for the development of new, more potent chemotherapies. I am hopeful 3-D/ECM assays become more embraced as a way to more accuratly determine in vivo cell behaviors.

Bioactive FGF Basic Recombinant Protein in Action

Maintaining Sheep Mesenchymal Stem Cell Multipotency

We have been very selective in the Bioactive FGF2 or FGF-basic Recombinant Proteins we make available to Stem Cell Researchers. They are many options available so we are always encouraged when we see ours referenced in publications.

Here researchers used our FGF2 to expand Sheep Bone Marrow Stem Cells (BMSCs) in culture: Troy D Bornes, Nadr M Jomha, Aillette Mulet-Sierra and Adetola B Adesida. Hypoxic culture of bone marrow-derived mesenchymal stromal stem cells differentially enhances in vitro chondrogenesis within cell-seeded collagen and hyaluronic acid porous scaffolds.Stem Cell Research & Therapy 2015, 6:84 doi:10.1186/s13287-015-0075-4.

These cells were used for chondrogenesis studies.

Expansion of BMSCs: Bone marrow aspirate collections containing 8 x 10⁷ MNCs were seeded within each 150-cm2 tissue culture flask. Culture medium composed of alpha-minimal essential medium (α-MEM) supplemented with 10% v/v heat-inactivated fetal bovine serum (FBS), penicillinstreptomycin-glutamine, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and sodium pyruvate (all from Life Technologies, Burlington, Canada) was pipetted into each flask. Fibroblast growth factor-2 (FGF-2; Neuromics Inc., Edina, USA) was added at a concentration of 5 ng/ml in order to maintain cell multipotency. Nucleated cells were allowed to adhere and grow for seven days before the first media change under normoxia (ambient 21% O2) or hypoxia (low 3% O2) at 37°C in a humidified incubator containing 5% CO2. Flasks from the hypoxic incubator experienced short periods (less than 5 minutes) of normoxic exposure during media changes. Thereafter, the media were changed twice per week until 80% cell confluence was obtained. Adherent BMSCs were detached using 0.05% w/v trypsin-ethylenediaminetetraacetic acid (EDTA; Sigma-Aldrich Corp., Oakville, Canada) and expanded under the same oxygen tension (normoxia or hypoxia) as during isolation until P2 prior to scaffold seeding. Hereafter for brevity, BMSCs described by expansion oxygen tension alone (normoxia-expanded and hypoxia-expanded BMSCs) will refer to BMSCs that were isolated and expanded under normoxia and hypoxia, respectively. The time taken from plating of nucleated cells (P0) to reach approximately 80% confluence at P2, before experimental use, varied from three to four weeks.

I anticipate more publications on these important bioactive reports as demand for them has been growing.

Markers for Oligodendrocyte Progenitor Cells (OPCs)

Improving OPC Expansion

Researchers reference use of Neuromics' GFAP and PDGF R Alpha/CD140A for OPC Selection

The data provided in this publication demonstrates that the OPC yield from SVZ-derived cell cultures can be improved with the PDGF-BB isoform in comparison to classical bFGF-EGF, or PDGF-AA-based protocols. Additionally, it would be expected that the OPC-enriched cultures obtained from NSC/NPC exposure to PDGF-BB and heparin generate cells suitable for cell transplantation for treating demyelinating diseases: Paula G. Franco, Juana M. Pasquini, Lucas Silvestroff. Optimizing Culture Medium Composition to Improve Oligodendrocyte Progenitor Cell Yields In Vitro from Subventricular Zone-Derived Neural Progenitor Cell Neurospheres. Published: April 2, 2015DOI: 10.1371/journal.pone.0121774:

Images: Detection of OPC markers by ICC on NS cells. A) Quantitation of NG2+ and/or PDGFRα+ cell proportions in WT mice NS generated in the presence of different growth factor combinations. Data belong to three independent experiments for each condition. Data for NG2-/PDGFRα- was analyzed with a One-way ANOVA plus Dunnett´s post test, where bFGF/EGF was set as the control. B, C) Representative images of NG2+ and PDGFRα+ cells generated from WT mice cultures in the presence of either bFGF/EGF or bFGF/PDGF-BB. D) Comparison of NG2+ or PDGFRα+ cell proportions in NS cultures generated from Act::EGFP mice in the presence of bFGF/EGF or bFGF/PDGF-BB. Data for NG2+ (dark magenta) or PDGFRα+ (light magenta) cells was analyzed separately with Student´s t test. E, F) NG2 and PDGFRα immunodetection in CNP::EGFP derived cultures. Gray bars in each graph were analyzed with Student´s t test. G) Olig2 expression in WT mice NS cultures. Asterisks are colour coded to indicate the pairs of bars compared and analyzed with Student´s t test. Cell proportions in A, and D-G are expressed as a fraction of the total cell nuclei counted for each condition. Error bars represent the SD for all bar graphs.. doi:10.1371/journal.pone.0121774.g001.

Neuromics has an excellent catalog of Stem Cell Selection Markers.

The Importance of in vivo Like Astroglial-Neuron Co-Cultures

What We Have Learned

I have many posting on both Neuron and Astroglial Cultures. We have now evolved our solution set to include in vivo like co-cultures. These Astroglial Neuron Co-cultures are designed to mimic in vivo like behaviors. They are potent, pure and proven to work in our clients' unique neurodegenerative disease and toxicity drug discovery assays.

Why is this important? The mix of Astrocytes, Glia and Neurons in your co-cultures can impact your data endpoints and lead to inaccurate conclusions. Here're examples as to how much your data can fluctuate. These are data generated from toxicity assays.

We stand ready to serve you and your team. Questions? Don not hesitate to call 612-801-1007 or e-mail me pshuster@neuromics.com. Pete Shuster, CEO and Owner, Neuromics.