Cancer Associated Fibroblasts (CAFs)-Flow Cytometry and Immunostaining Results

If they Walk and Talk Like CAFs, They Are!

We are experiencing growing demand for are CAFs. They are closely associated with primary tumor cells and participate in the neoplastic process. There is reciprocal communication between CAFs and tumor cells through paracrine effects of secreted growth factors, cytokines & chemokines from both fibroblasts, tumor cells and other tumor-associated cells.

Potential users are asking how well the cells are characterized. The purpose of this posting is to share key gene expression data:

CELL LINES	CD105	CD90	CD44	CD326	CD133	FAP
SC00A1 (hMSCs)	+	+	+	-	-	-
SC00A5 (pancreatic fibroblasts)	+	+	+	-	-	-
CAF05 (colorectal CAFs)	+	+	+	-	-	+
CAF07-AD (adenocarcinoma lung CAFs)	+	+	+	-	-	+
CAF08 (Pancreatic Stellate CAFs)	+	+	+	-	-	+

Table: expression of key proteins

Images: Human Lung Adenocarcinoma CAFs stained with Vimentin, aSMA, b-Catenin and DAPI (blue),

Cancer Associated Fibroblasts Gene Expression Data(pdf - 784Kb)-Flow Cytometry and Immunostaining Results.

Questions? Please contact me directly-Pete Shuster- CEO & Owner-pshuster@neuromics.com or 612-801-1007.

The Growing Value of Cancer Associated Fibroblasts or CAFS

Balancing Supply and Demand

There is reciprocal communication between Cancer Associated Fibroblasts (CAFs) and tumor cells. They are now recognized as playing a key role in promoting the malignant process and blocking immune surveillance  They are becoming increasing important in Cancer Drug Discovery Programs.

Their growing value is making it harder for us to source resected tumors. We have been receiving advanced orders from clients who need to insure they have a stock. We have ordered tumors for the below CAFs and will be shipping to those who have place advance orders by the end of November.

Name	Catalog #	Type	Species	Applications	Size	Price
Human Colorectal Tumor CAFs Kit	CAF05	Human Primary CAFs	H	Cell Assays	1,000,000 cells	$799
Human Endometrial Adenocarcinoma CAFs	CAF01	Human Primary CAFs	H	Cell Assays	1,000,000 cells	$799
Human Ovarian Serous Tumor CAFs	CAF02	Human Primary CAFs	H	Cell Assays	1,000,000 cells	$799
Human Lung Adenocarcinoma CAFs	CAF07A	Human Primary CAFs	H	Cell Assays	1,000,000 cells	$799
Human Lung Squamous Cell Tumor CAFs	CAF07SC	Human Primary CAFs	H	Cell Assays	1,000,000 cells	$799
Human Immortalized Pancreatic CAF-Stellate Cells	CAF08	Human Primary CAFs	H	Cell Assays	500,000 Immortized Cells	$4,335
Human Pancreatic Tumor Cells Kit	PXPC3A1	Cells+Media Kit	H	Cell Assays	1,000,000 cells	$499
Conditioned Media for Ovarian Serous Tumor CAFS	CAF04	Cell Growth Media	H	Cell Assays	5 ml	$250
Conditioned Media from Endometrial CAFS	CAF03	Cell Growth Media	H	Cell Assays	5 ml	$250
VitroPlus III, low serum, complete	PC00B1-100	Cell Growth Media	H	Cell Assays	100 ml 500 ml	$65 $165

Image: Endometrial CAFs courtesy of Tiana Tonrey, Vitro Biopharma

Should have questions about availability and ship dates for any of these please call (612-801-1007) or e-mail: pshuster@neuromics.com. Thank you.
Pete Shuster, CEO, Neuromics