Schwann Cell-Sensory Neurons-PNS Markers

Data-Publications
These markers a important tools for the study of Neuro-muscular diseases like Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS).

For Neuromuscular Disease Researchers, we have some of the best Schwann Cell and Sensory/Peripheral Neuron Markers in the business.

Images: Rat mixed neuron/glial cultures stained with Peripherin (green channel) and Neurofilament alpha-internexin/NF66 (green channel). These cultures contain mostly neurons which are rich in alpha-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of peripherin and alpha-internexin, the green and red signals superimpose to produce a golden cell. Blue is a DNA stain. Protocol on data-sheet.

Here're recent publications referencing use of these markers:
Leah R. Reznikov, Qian Dong, Jeng-Haur Chena, Thomas O. Moninger, Jung Min Park, Yuzhou Zhang, Jianyang Du, Michael S. Hildebrand, Richard J. H. Smith, Christoph O. Randak, David A. Stoltz, and Michael J. Welsh. CFTR-deficient pigs display peripheral nervous system defects at birth. www.pnas.org/cgi/doi/10.1073/pnas.1222729110...goat anti-p75 (1:500; Neuromics)...

Gayle M. Passmore, Joanne M. Reilly, Matthew Thakur, Vanessa N. Keasberry, Stephen J. Marsh, Anthony H. Dickenson and David A. Brown. Functional significance of M-type potassium channels in nociceptive cutaneous sensory endings. Fronteirs in Molecular Science. doi: 10.3389/fnmol.2012.00063. ...neurofilament H (1:1000,Neuromics,USA)...

Leigh A Nattkemper, Zhong-Qiu Zhao, Anna J Nichols, Alexandru D P Papoiu, Carol A Shively, Zhou-Feng Chen and Gil Yosipovitch. Over-Expression of the Gastrin-Releasing Peptide in Cutaneous Nerve Fibers and its Receptor in Spinal Cord in Primates with Chronic Itch. Journal of Investigative Dermatology accepted article preview 4 April 2013; doi: 10.1038/jid.2013.166...Protein Gene Product 9.5 (PGP9.5; Neuromics, Edina, MN).

Wiebke Kallenborn-Gerhardt, Katrin Schröder, Domenico Del Turco, Ruirui Lu, Katharina Kynast, Judith Kosowski, Ellen Niederberger, Ajay M. Shah, Ralf P. Brandes, Gerd Geisslinger, and Achim Schmidtko. NADPH Oxidase-4 Maintains Neuropathic Pain after Peripheral Nerve Injury. The Journal of Neuroscience, 25 July 2012, 32(30): 10136-10145; doi: 10.1523/​JNEUROSCI.6227-11.2012...chicken anti-P-Zero or MPZ (1:500; Neuromics)...

Images: Micrographs depicting SSeCKS colocalization with myelination markers (CNPase and Pzero). (A) SSeCKS (red) and CNPase (green) in the lumbar spinal cord dorsal horn. The labeling appears discrete with minimal colocalization. (B) SSeCKS (red) and Pzero (green) in the L4 dorsal root ganglia. A lack of co-localization is observed and Pzero can be seen localized to putative axonal elements (arrow). (C) SSeCKS (red) and Pzero (green) in the sciatic nerve. As in the dorsal root ganglia, a lack of co-localization is observed. Both SSeCKS and Pzero can be seen localized to axonal elements. (D) SSeCKS (red) and Pzero (green) in glabrous skin of the hind-paw, fibers displaying colocalization (yellow) can be observed (arrow). Irmen et al. Journal of Brachial Plexus and Peripheral Nerve Injury 2008 3:8 doi:10.1186/1749-7221-3-8.

I will post new data and pubs as they become available.

Schwann Cells Markers

Important tool for studying diseases that cause PNS abnormalities or degeneration.

It is a goal of mine to have the best and brightest collection of Neuron-Glial Markers. Here I feature use of one of our Schwann Cell Markers. In this study our p75/NGF antibody is used as a control to determine differences in myelin sheath structure in new born pigs having the Cystic Fibrosis Mutant vs Normal Gene Expression (controls). Leah R. Reznikov, Qian Dong, Jeng-Haur Chena, Thomas O. Moninger, Jung Min Park, Yuzhou Zhang, Jianyang Du, Michael S. Hildebrand, Richard J. H. Smith, Christoph O. Randak, David A. Stoltz, and Michael J. Welsh. CFTR-deficient pigs display peripheral nervous system defects at birth. www.pnas.org/cgi/doi/10.1073/pnas.1222729110.

Images: CFTR is functionally active in Schwann cells. (A) Primary cultures of porcine Schwann cells were used 4 wk after seeding when they had developed the specific bipolar morphology and a phase-bright cell body under differential interference contrast microscopy. Schwann cells were positive for the phenotypic markers S100 and p75. (B) Whole-cell current recorded in the presence of PKA and ATP in the pipette solution and 1 min after adding 100 μM of CFTR inhibitor GlyH-101 to the bath solution. (Left) Example of currents from one cell; Inset shows voltage-pulse protocol. (Upper Right) Example of current-voltage relationship. (Lower Right) Data from five CFTR+/+ Schwann cells and seven CFTR−/− Schwann cells. *P = 0.003 (Mann–Whitney rank sum test).

Images: Fig. 1. CFTR is expressed in trigeminal nerve Schwann cells. Data are confocal microscopic images of trigeminal nerve immunostained for CFTR (green) and marker indicated above middle panels (red). Nuclei were stained with DAPI (blue). (A) Transverse cross-section with axons stained with β-tubulin III antibody. (B) CFTR−/− trigeminal nerves immunostained for CFTR and β-tubulin III.  (C) Sagittal crosssection immunostained with fluoromyelin. (D) Section stained with S100 antibody, a marker of Schwann cells. (E) Section stained with p75 antibody, also a marker of Schwann cells. (Scale bar, 20 μm.).

I will continue to post new customer pubs and other shared customer data featuring use of our Neuron-Glial Markers.