Human Brain Cells

Our Expertise
When researchers are considering using our human brain cells, they often voice concerns about growth. In order to address these concerns, we explain that we have optimized our media and coatings in order to meet their assay needs.

Here's an example of how our human brain pericytes grow over time.

We have primary human neurons, astrocytes, microglia and Schwann cells.

If you have interest you can contact us at 866-350-1500 or rose@neuromics.com. We will provide the information required to help you make an informed decision.

"Hard to Find" Cells

You can Trust Your Results
Cells form the foundation of your cell based assays. We work hard to provide the cells you need and, more importantly that they are healthy and happy.

We are pleased to announce that we are recognized for having primary human cells that comply with federal and international regulations. This is especially important in drug discovery, because if the cells yield targets that result in future therapies this validation becomes increasingly valuable.

Human Brain Microvascular Endothelial Cells in Culture

Looking for "hard to find cells"? Check us out today.

Cancer-Associated Fibroblasts (CAFs) and Cells

Solutions for studying tumor growth, angiogenesis and metastasis.

Human Brain Astrocytes-New

Derived from Cortex

Immortalized Human Brain Microglia Cells

Isolated from healthy human brain tissue

Human Schwann Cells - New

Human spinal nerve derived primary cells

hN2™ Neuron Discovery Kits

Derived from H9 (WA09) ECSs-Consistent, Easy to Use & Cost Effective

Human alpha Motor Neurons Kit

hMSC Derived-includes 100 ml of media to optimize your cultures

Human Cardiomyocytes-New

Derived from hMSCs

Human Chondrocytes-New

Derived from hMSCs

Human Endothelial Cells-New

Artery, Microvascular, Vein & Umbilical Cord Derived + Culture Media

Human Fibroblasts-New

Pancreas and Neonatal Dermal Derived

NeuroNet Pure Human Neurons Discovery and HTS Kits

Pure Population of hPSC Derived Neurons

Human Osteoblasts-New

Derived from hMSCs

hMPro™ Human Mesenchymal Progenitors (hMPCs)

hESC Derived

Human Mesenchymal Stem Cells (hMSCs)

hMSCs Derived from Umbilical Cord Blood

Stressed Selected Stem Cells

They Survive to Thrive

hAstroPro Human Astrocyte+Astroglial-Neurons Co-Culturing Kits

Pure, Potent and Proven

Human Pericytes

Brain and Retinal Derived

3-D Cell Based Assay Solutions

Nanofibers, Hydrogels and Extracellular Matrix (ECM) Proteins

Human alpha-Sensory Neurons

Put Them to Work!

We continue to add unique primary Human Neurons to our offerings.

We have discounted our new alpha-Sensory Neurons to make it easier to buy and try-only 850 USD/1,000,000 cells.

Just thaw and culture. Simply culture on ECM- Laminin or Fibronectin coatings with our alpha Motor Neuron Maintaining Media. Questions? Please contact me directly 612-801-1007 or pshuster@neuromics.com. Pete Shuster, CEO and Owner, Neuromics.

Neurotrack Player, Essen Bioscience & Neuromics Neurons

I claim that our solutions are thoroughly tested, validated and research ready. We measure our performance by making sure this claim rings true with our customers.

One way this is confirmed to me is when a partner has success using one of our solutions in a new way. With that I am pleased to update you on Essen Bioscience's application for our primary neurons. Please note the related assays are being demonstrated in their most current webinar.

We will be featuring kinetic and migration assays using our hMSCs, hOsteoblasts and hChondrocytes in the near future. So stay tuned.

hN2 Human Neurons for Toxicity Screening

Our Human hN2 Neurons proving excellent platforms for Neurotoxicology Studies. I would like to share recent publication and that confirms the potential of these solutions for use in your toxicology assays.

Xiugong Gao, Hsiuling Lin, Radharaman Ray, Prabhati Ray. Toxicogenomic Studies of Human Neural Cells Following Exposure to Organophosphorus Chemical Warfare Nerve Agent VX. Neurochemical Research. February 2013.

Abstract: Organophosphorus (OP) compounds represent an important group of chemical warfare nerve agents that remains a significant and constant military and civilian threat. OP compounds are considered acting primarily via cholinergic pathways by binding irreversibly to acetylcholinesterase, an important regulator of the neurotransmitter acetylcholine. Many studies over the past years have suggested that other mechanisms of OP toxicity exist, which need to be unraveled by a comprehensive and systematic approach such as genome-wide gene expression analysis. Here we performed a microarray study in which cultured human neural cells were exposed to 0.1 or 10 μM of VX for 1 h. Global gene expression changes were analyzed 6, 24, and 72 h post exposure. Functional annotation and pathway analysis of the differentially expressed genes has revealed many genes, networks and canonical pathways that are related to nervous system development and function, or to neurodegenerative diseases such as Alzheimer’s disease, Huntington’s disease, and Parkinson’s disease. In particular, the neuregulin pathway impacted by VX exposure has important implications in many nervous system diseases including schizophrenia. These results provide useful information valuable in developing suitable antidotes for more effective prevention and treatment of, as well as in developing biomarkers for, VX-induced chronic neurotoxicity.

Images: hN2 Neurons at 18, hrs, 72 hrs and 6 Days.

Image: hN2 cells grown in culture for 4 days and stained with our chicken polyclonal to Neurofilament light or low molecular weight chain NF-L, a marker of neurons. Many of the differentiating cells show strong cytoplasmic and clearly fibrillar staining for NF-L. Blue stain is DAPI and reveals cell nuclei of some non neuronal cells in this culture

I will continue to post updates on new applications of these potent, pure and easy to grow human neurons.

Primary Neuron Assays for Studying Neurodegeneration

Our goal is to provide our customers and collaborators the tools they need to insure success. This is defined by having the specific Primary Neurons, Growth Factor plus the Markers to meet unique research needs.

The proof is in the results. Here are some highlights.

Images/Data: FIGURE 5. Microglial p38α MAPK-dependent TNFα is involved in LPS-induced neurite degeneration. (A) Photomicrographs of MAP-2 immunocytochemistry show the morphology of neurons after 72h of co-culture with microglia. The arrow points to the appearance of neurites that have been damaged by LPS-activated WT microglia. In contrast, the arrowhead points to the morphological appearance of healthy, undamaged neurites. (B) Diagram of the Sholl method for quantifying the total number of healthy neurites that intersect the concentric circles. (C) Quantification of healthy neurites by the Sholl analysis demonstrates that LPS stimulation of p38α WT microglia in co-culture causes neurite degeneration as seen by a significant reduction in the number of intersections by healthy neurites in the LPS-stimulated group compared to the unstimulated group (white bars). This degeneration can be attenuated by the addition of a blocking antibody to TNFα (5μg/ml), while the non-immune IgG control was not protective (gray bars). Microglia from p38α KO mice stimulated with LPS (black bar) also have significantly less neurite degeneration than the LPS-stimulated p38α WT microglia (white bar). However, by adding TNFα back to the p38α KO microglia co-culture, there is a significant decrease in the healthy neurite arborization compared to the p38α KO microglia stimulated with LPS alone (black bars). (***p<0.005; Bonferroni’s multiple comparison test). Data represents 2 independent experiments. Scale bar equals 25μm. Molecular Neurodegeneration 2011, 6:84 doi:10.1186/1750-1326-6-84

hN2 cells grown in culture for 4 days and stained with our chicken polyclonal to Neurofilament light or low molecular weight chain NF-L, a marker of neurons. Many of the differentiating cells show strong cytoplasmic and clearly fibrillar staining for NF-L. Blue stain is DAPI and reveals cell nuclei of some non neuronal cells in this culture.

We will continue to post relevant images and data that demonstrate our capabilities.

Transfection/Infection of Primary Neurons

Gene Expression Analysis of Neurons is an important tools in basic research and the study of neuropathologies. At the Neuromics' blog: "siRNA, DsiRNA and Plasmid Transfection Efficiency", I have posted many examples of successful tarnsfection of primary neurons and related cells using both our Transfection Kits/Reagents and others.

The other puzzle piece for these studies is having a fresh, pure and easy to use source of cells. Here, Neuromics has many options. These primary neurons and neural progenitors are widely referenced in key publications. Applications referenced include: transfection, pharmacology, electrophysiology, immunocytochemistry, and neuronal development studies.

This posting features infection of our e18 Primary Rat Combined Hippocampus, Cortex, and Ventricular Neurons using Nipah virus related components and HeV pseudotyped virions

Aparna Talekar, Antonello Pessi, and Matteo Porotto. Infection of primary neurons mediated by Nipah virus envelope proteins: Role of host target cells in antiviral action. J. Virol. doi:10.1128/JVI.00452-11.

Abstract: We have previously described heterotypic peptides from parainfluenza virus that potently inhibit Nipah virus in vitro, but are not efficacious in vivo. By contrast, our second-generation inhibitors, featuring a cholesterol moiety, are also efficacious in vivo. The difference between in vitro and in vivo results led us to investigate the basis for this discrepancy. Here we compare the activity of the compounds in standard laboratory cells and in cells relevant to the natural tropism of Nipah virus, i.e. primary neurons, and show that while our first generation inhibitors are poorly active in primary neurons, the cholesterol-conjugated compounds are highly potent. These results highlight the advantage of evaluating antiviral potency in cells relevant to natural host target tissue.

Customer Data: Transfection of functional HeV glycoproteins and infection with HeV pseudotyped virions.In order to establish the feasibility of carrying out the proposed experiments in primary neurons, we show (figure ) that our assays are amenable to use in primary neurons. In the experiment, Combined Hippocampus, Cortex, and Ventricular -E18 (Neuromics) were plated, and at 3 days were transfected with plasmids encoding HeV G/F as well as YFP. On the following day, these cells were infected with HeV or VSV pseudotyped viruses bearing RFP. In the figure, (A) the phase contrast photos show the differentiated neurons; (B) upon excitation for RFP, the red fluorescence indicates neurons infected by HeV pseudotyped virions; (C) upon excitation for YFP, and the green fluorescence shows the efficiency of transfection in neurons. This experiment indicates that the proposed experiments can be carried out in primary neurons, which are transfectable and infectable in our systems, and thus supports all the proposed aims. Data Courtesy of Dr. Matteo Porotto, Weill Cornell Medical College. Larger Image

We will continue to keep you updated.

H9-WA09 Derived Human Neural Progenitors and Neurons Pricing

Neuromics offers stable, potent and well characterized STEMEZ (TM) Human Neural Progenitor & Neuron Discovery Kits. These are derived from NIH registered human ES cell line H9 (WA09).

Details on the capabilities of these cells are detailed in a variety of publications. They work! As a result, I am trying to make it easier to justify using them when and where they are needed. In addition to having frequent updates on new references, methods and data, I want to offer the best pricing possible.

In researching pricing of cells derived from the same parental line and having similar characteristics, I noticed prices ranging from 995 to 2800 USD. Our pricing starts at 695 USD and we offer deeper discounting if required. I do not want price to be a barrier. Here's some sample data on these cells.

Images: Neural phenotypes derived from hN2 cell lines. (A) Phase contrast image of differentiated culture. (B) Network including post-mitotic motoneurons (HB9). (C) Cholinergic neuron. (D) Tuj-1 positive cells that are DAT-positive (dopamine transporter; closed arrow) and DAT-negative (open arrow). (E) Gabaergic neurons, inset illustrates GABA in axon, but not the dendrites (arrow).

G-protein Coupled Receptor Expression Patterns Are Altered as Human Embryonic Stem Details-From Poster Presented at Neuroscience 2008 by Dr. Steve Stice et al.
hN2 Cells-Electro Phys Data.

Related Products:

• All Stem Cell Research Reagents
• Primary Neurons and Astrocytes

I wish exciting and rewarding discoveries.