FBS Works Great for Cell Lines

More Customer Data
We are pleased with the continuing stream of positive feedback on our potent FBS. This includes its supplementing media for cell lines.

Here is an image courtesy of  Kavita Shah of Purdue University.

Image: HEK Cells cultured in Media Supplemented with Neuromics' FBS

We offer the best price anywhere. We will beat all competitive pricing. If you are interested, contact Rose Ludescher, Manager of Customer Satisfaction. email: rose@neuromics.com.

Data Trifecta-Cells, Media + FBS

Optimizing your Cell-Based Assays
Researchers need solid proof that the solutions the use will work in their assays. Given the time and effort it takes to culture human primary cells, it is important that the media and supplements used are optimized.

We engineer our cell media and supplements to work with our primary and stem cells. Its potency is tested every day in our cell cultures.

As further proof, we ask customers to share their data with us. We reward them by emailing a 50 USD Amazon gift card. I would like to recognize Emily Rodela from TGEN for sharing the Trifecta of Neuromics' CAFS grown in MSC-Gro Media supplemented with our FBS.

Image: Human CAFS-Pancreatic Stellates cultured in MSCGro™ supplemented with Neuromics' FBS - Fetal Bovine Serum. Courtesy of Emily Rodela, TGEN.

Neuromics' ISOKine bFGF in 3-D Cultures

Works Well in Perfusion Models

Our ISOKine^TM FGF is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.

It is a proven solution for all cell cultures and starts at the low price of  65 USD/10 ug.

Here's a reference of its use in 3-D Cultures: Tom Kamperman, Sieger Henke, Claas Willem Visser, Marcel Karperien, Jeroen Leijten. Centering Single Cells in Microgels via Delayed Crosslinking Supports Long-Term 3D Culture by Preventing Cell Escape. DOI: 10.1002/smll.201603711.

Figure: Delayed on-chip crosslinking enables centering of single cells in microgels. a) Fluorescence confocal imaging confirmed that delayed enzymatic crosslinking enabled centering of single MSCs in Dex-TA microgels. b) On average, cell-laden microgels were only 9 µm larger than the encapsulated MSCs, effectively resulting in 3D hydrogel coatings of less than 5 µm. c) A standard microfluidic droplet generator was connected to the H2O2 diffusion-based crosslinking chip. The position of cells (white arrows) in non-crosslinking microgel precursor droplets was analyzed d) immediately after droplet generation (t1), at the start of the crosslinking chip (t2), and e) at the end of the crosslinking chip (t3). f) Cell positions within microgels produced using conventional microfluidic encapsulation systems (i.e., with coupled emulsification and gelation) are indicated with gray (i.e., references) and red (i.e., this work) data points. Cell positions within gel precursor droplets along the modular microfluidic setup are indicated with blue data points. Cell positions within delayed enzymatically crosslinked microgels are indicated with green data points. g) Cell position analyses of various combinations of distinct hydrogel materials (i.e., Dex-TA, Dex-HA-TA, PEGDA), cell types (i.e., MIN6, MSC), and crosslinking methods (i.e., enzyme-based and photo-crosslinking), revealed that delayed crosslinking consistently resulted in significantly increased cell-centering as compared to the conventional encapsulation approach where emulsification and gelation are coupled.

If you are looking for competitively priced, animal free and potent growth factors. check out our ISOkines.

Cancer Associated Fibroblasts in Action

Publication Reference
We introduced our Cancer Associated Fibroblasts to researchers several years ago. The demand was strong from day one and continues to grow.

We are pleased to announce the first reference in a Cancer Research Publication: Zenobia D'Costa, Keaton Jones, Abul Azad, Ruud van Stiphout, Su Y Lim, Ana L Gomes, Paul Kinchesh, Sean C Smart, W. Gillies McKenna, Francesca M Buffa, Owen J. Sansom, Ruth J. Muschel, Eric O'Neill and Emmanouil Fokas. Gemcitabine-induced TIMP1 attenuates therapy response and promotes tumor growth and liver metastasis in pancreatic cancer. Published OnlineFirst August 1, 2017 doi: 10.1158/0008-5472.CAN-16-2833...Immortalized Pancreatic CAF-Stellate Cells were obtained from Neuromics, and cultured in VitroPlus III, low serum, complete (Neuromics)...

Image: Pancreatic CAF-Stellates stained with alphaSMA

We will continue to post new developments here.

Pain and Inflammation Markers

Our TRPV1 Markers Rock
As sales of our Cell Based Assay Solutions continue to explode. We don't want you to forget that our roots firmly remain in products for Inflammation and Pain Researchers.

To verify this, you only need to check out the many publications referencing use of our TRPV1 Markers.

Image: Immunoreactivity for CXCL12 was detected in the macrophages (F4/80)