50 USD Reward
Our
Customer Data program is progressing nicely. I am pleased to present the latest addition from Dr. Vimal Swarup, University of Utah. To qualify for the reward just email Rose Ludescher-
rose@neuromics.com.
Hippocampalneuron-based guidance assay. Image of micro-contact patterned chondroitin
sulfate proteoglycans (pre-mixed with AlexaFluor 594) purified from astrocyte
conditioned media (CM). b) Immuno-stain of the spot in (a) with anti-CSPG
antibody CS56. c) Overlay of Embryonic day-18 primary rat hippocampal neurons
(Cat# PC35101, Neuromics Inc.) and the underlying surface containing μC
patterned CM after 48 h of growth. d) Overlay of neurons and underlying surface
containing μC patterned CSPGs purified from CM. e) Average neurite length on
pLL and stamped regions after 48 h. f) Average neurite length on plain field
surfaces of μC patterned CM, CSPG and pLL after 48 h. Results from this
experiment show that neurons avoid CM or CSPGs purified from CM while
elongating.
Swarup et al. JACS. 2013
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