Detecting Expression of CAS9

Gain Trust That Your Gene Editing is on Target CRISPR/Cas9 is a hot genome-editing technology. However, the high frequency of off-target activity is a major issue.
One way to make sure CAS9 is being properly expressed is using markers against related proteins.

We are please to introduce our CAS9 markers. We plan on adding to these in the near future.

Image: Transfected Hek293 cells overexpressing the N-terminal 1-608 amino acids of S. pyogenes Cas9 . The cells were stained with MO22174 in red, and these cells also appear yellow since we counterstained with our rabbit Cas9 antibody, RA22126 to the same construct in green, giving a yellow color. The N-terminal construct contains a nuclear localization sequence and so is predominantly nuclear in localization. Most Hek293 cells in this field are not transfected so only the nuclei of these cells can be visualized with the blue DAPI DNA stain.

Name	Catalog #	Type	Applications
Cas9 from Steptococcus pyogenese	MO22174	Mouse IgG	ICC; IF; WB
Cas9 from S. pyogenese	RA22126	Rabbit IgG	ICC; IF; WB
Cas9 from Staphylococcus aureus	MO22175	Mouse IgG	ICC; IF; IHC; WB

We will post new CAS9 marker additions here.