Nestin Expression in Differentiating Neural Progenitors

Breathtaking Images

Our Nestin Antibody + a ​HES5::eGFP reporter was one of the markers used to visualize differentiating stem/progenitor cells (see: Nature Communications 6, Article number: 6500 doi:10.1038/ncomms7500).

Figures: (a) Neural differentiation scheme. Neural induction was performed by a dual SMAD inhibition protocol followed by long-term propagation with the factors indicated for 220 days. Naming conventions representing neuroepithelial (NE), early radial glial (E-RG), midradial glial (M-RG), late radial glial (L-RG) and long-term cultured progenitors (LNP) are indicated. Number of passages are indicated as P(n). (b) Bright field microscopy of progenitor cells during long-term differentiation shows dynamic morphological features. Scale bar: 50 μm (valid for all images in b). (c) Combined ​HES5::eGFP reporter expression and Nestin Immunostainings of stem/progenitor cells.

We have a large catalog of  Neuronal-Glial Markers. They are research proven and frequently published. Should have questions do not hesitate to call me directly 612-801-1007 or pshuster@neuromics.com. Pete Shuster, CEO and Owner.

Neuro-Toxicity Co-Culture

Poster Presentation at Society of Toxicologist Annual Meeting 2015.

As part of Neuromics' strategic selling partnership with ArunA Biomedical. We are attending the SOT 2015 meeting in San Diego, CA.

I am particular excited about our getting the word out on our solutions via poster presentations. Here's the line up:

“In-vitro Human Developmental Neurotoxicity Screening Using Multiple Cell Types"

Anirban Majumder¹, Xian Wu^2,3, Shelley Wallace¹, Jane Le¹, Steven L. Stice^1,2,3

 ¹ArunA Biomedical, Inc. Athens, GA, USA, ²Regenerative Bioscience Center, ³Interdisciplinary Toxicology Program, University of Georgia, Athens, GA, USA

Abstract Number/Poster Board number: 636 Poster Board -443

Presentation: March 23, 2015 1:00 PM to 4:30 PM, CC Exhibit Hall 

“Mouse Pluripotent Stem Cell Motor Neurons Generate Robust Neural Network Activity on Microelectrode Arrays"

Steven L. Stice^1,2 Anirban Majumder¹, Brad Culp¹, Anthony M. Nicolini³ and Colin Arrowood³

 Aruna Biomedical Inc. ¹, Regenerative Bioscience Center, Univ. of Georgia, Athens GA. ², Axion BioSystems, Atlanta, GA³

Abstract Number/Poster Board number: 279 Poster Board - 450

Presentation: March 23, 2015 from 9:00 to 12:00 CC Exhibit Hall

 “Bisphenol-A effects on in vitro Human Neural Development was Window of Susceptibility dependent"

Xian Wu^1,2, Anirban Majumder³, Steven L. Stice^1,2,3

¹Interdisciplinary Toxicology Program, ²Regenerative Bioscience Center, University of Georgia, Athens, GA, USA  ³ArunA Biomedical, Inc. Athens, GA, USA  

Abstract Number/Poster Board number: 249 Poster Board - 415

Presentation: March 23, 2015 from 9:00 to 12:00 CC Exhibit Hall

“MR Imaging of Human Neural Progenitor Stem Cells: An In Vivo Longitudinal Model "

Forrest Goodfellow, Qingying Ming, Xian Wu, Erin Jordan, Qun Zhao, Steve Stice

Interdisciplinary Toxicology Program, University of Georgia, Athens, Georgia 30602

Abstract Number/Poster Board number: 641 Poster Board -448

Presentation: March 23, 2015 from 1:00 PM to 4:30 PM CC Exhibit Hall 

"Using Human-Derived Neural Cells As an In Vitro Model for Developmental Neurotoxicity following Exposure to Pesticides"

Mary Smith^1,2 , Mayowa Amosu¹ , Xiaoming Bian¹ , Kun Lu¹ , Steven Stice^2,4 , William Henderson³ , Shelley Wallace⁴ Anirban Majumder⁴

¹Department of Environmental Health Science, University of Georgia, Athens, Georgia, United States; ² Regenerative Bioscience Center, University of Georgia, Athens, Georgia, United States; ³ ORD/NERL/ERD, U.S. EPA, Athens, Georgia, United States; ⁴ ArunA Biomedical, Inc., Athens, Georgia, United States

Abstract Number/Poster Board number: 1747 Poster Board - 152

Presentation: March 23, 2015 from 9:00AM  to 12:00 PM 

More to follow...

The Power of ISOkine Stem Cell Growth Factors

What Endotoxin, Animal and Serum Free Means to You

ISOkine™ growth factors are produced in barley, bypassing the use of bacterial or animal cell systems.

Why Does this Matter? Proteins produced in bacterial systems contain trace Endotoxins (toxins produced by the bacterial hosts). Studies show that these Endotoxins can compromise health of your stem cell based assays. Animal and human cell expression systems pose risk to your assays, because they may harbor pathogens.

They are Low Cost and Work in the Hands of our Customers: "The ISOKine mouse LIF is an excellent product! I enjoyed dealing with Neuromics. My interactions with Brett were very professional and he assured me the product was guaranteed to work; which it did." Andy Babwah, Children’s Health Research Institute

Product Options:

Name	Catalog #	Type	Species	Size	Price
ISOKine-EGF	PR80002-100	Protein	H	100 ug	$99
ISOKine-Flt3-Ligand, human-NEW	PR80004-10	Protein	H	10 ug 100 1 mg	$129 $529 $2,649
ISOKine-bFGF	PR80001-10	Protein	H; M	10 ug 50 ug 100 ug	$65 $169 $225
ISOKine-Leukemia Inhibitory Factor (LIF), human-NEW	PR80003-10	Protein	H	10 ug 100 ug 1 mg	$90 $395 $2,475
ISOKine-Leukemia Inhibitory Factor (LIF), mouse-NEW	PR80000-10	Protein	M	10 ug 50 ug 100 ug 1 mg	$90 $250 $395 $2,475
ISOKine-SCF, human-NEW	PR80005-10	Protein	H	10 ug 100 ug 1 mg	$90 $395 $2,475

Should have questions do not hesitate to call me directly 612-801-1007 or pshuster@neuromics.com. Pete Shuster, CEO and Owner.

Curcumin and Aging Health

Cellular Uptake Matters-Not All Curcumin Based Supplements Are Created Equal

There have been many academic publications on the miracles of Curcumin for Aging Health. Here's a description of its therapeutic properties: The desirable preventive or putative therapeutic properties of curcumin have also been considered to be associated with its antioxidant and anti-inflammatory properties. Because free-radical-mediated peroxidation of membrane lipids and oxidative damage of DNA and proteins are believed to be associated with a variety of chronic pathological complications such as cancer, atherosclerosis, and neurodegenerative diseases, curcumin is thought to play a vital role against these pathological conditions. The anti-inflammatory effect of curcumin is most likely mediated through its ability to inhibit cyclooxygenase-2 (COX-2), lipoxygenase (LOX), and inducible nitric oxide synthase (iNOS). COX-2, LOX, and iNOS are important enzymes that mediate inflammatory processes. Improper upregulation of COX-2 and/or iNOS has been associated with the pathophysiology of certain types of human cancer as well as inflammatory disorders. Because inflammation is closely linked to tumor promotion, curcumin with its potent anti-inflammatory property is anticipated to exert chemopreventive effects on carcinogenesis. Hence, the past few decades have witnessed intense research devoted to the antioxidant and anti-inflammatory properties of curcumin. In this review, we describe both antioxidant and anti-inflammatory properties of curcumin, the mode of action of curcumin, and its therapeutic usage against different pathological conditions see: (Adv Exp Med Biol. 2007;595:105-25).
Read More,,,

The Roots of Anxiety Induced Pain

Corticotropin-Releasing Factor and ERK1/2 Pathway

This study crossed my radar scope because the investigators referenced use of our phosphoERK1/2 for Western Blotting and Immunohistochemistry: Gisela Patrícia da Silva Borges , Juan Antonio Micó Segura , Fani Lourença Moreira Neto , Esther Berrocoso. Corticotropin-Releasing Factor Mediates Pain-Induced Anxiety through the ERK1/2 Signaling Cascade in Locus Coeruleus Neurons. DOI: http://dx.doi.org/10.1093/ijnp/pyv019 First published online: 25 February 2015

Conclusion: pain-induced anxiety is mediated by CRF neurotransmission in the LC through ERK1/2 signaling cascade.

Figure: a) Schematic representation of the anatomical pathways implicated. Briefly, the contralateral LC indirectly receives inputs from the inflamed paw (red dashed line; ascending pathways) and, subsequently, the information is sent to corticolimbic areas. Additionally, the LC sends direct projections to the spinal cord (blue straight line; descending pathways). b) Body weight of the control and MA rats. c) Body rectal temperature of control and MA rats. d) Mechanical hyperalgesia represented by a significant decrease in the paw withdrawal threshold of the ipsilateral paw of MA rats. e) Mechanical allodynia represented by a significant decrease in the force threshold of the ipsilateral paw of MA rats. Graph depicting the expression of pERK1/2 in the LC after intra-LC administration of the αCRF receptor antagonist, showing that the significant increase of pERK1/2 in MA4W animals was no longer observed when this antagonist was administered.  g) Graph showing that the local administration of the αCRF antagonist had no significant effect on mechanical hyperalgesia in MA4W rats. h) Graph showing that local administration of h) Graph showing that local administration of the α-helical CRF antagonist had no significant effect on mechanical allodynia in the ipsilateral paw of MA rats. i) Graph showing that the time spent in the open arms decreased in MA4W rats receiving the vehicle alone but this effect was successfully reversed by administration of the αCRF antagonist. j) Graph showing that local administration of the α-helical CRF antagonist had no significant effect on the total distance traveled in the elevated zero maze. k) Graph showing that local administration of the α-helical CRF antagonist reversed the decrease in the number of entries into the open arms observed in MA4W rats receiving the vehicle alone. B=Baseline; LC=Locus Coeruleus; αCRF=antagonist of the corticotropin-releasing factor receptor I and II; W=Week; MA=Monoarthritis.

 



Western Blotting: The membranes were blocked with 5% Bovine Serum Albumin (BSA; Sigma, Spain) in TBST and probed overnight at 4 ºC with a rabbit anti-phospho-ERK1/2 (1:5,000; Neuromics). Immunohistochemistry: Brains were removed and processed for free-floating immunohistochemistry. One in five sequential transverse brain sections (30 µm) containing the PVN from each rat were washed, blocked and incubated with a rabbit antiserum against the phosphorylated ERK1 and ERK2 isoforms (pERK1/2; 1:1000; 48 hours at 4-8ºC: Neuromics, USA). Immunodetection was achieved with a biotinylated donkey anti-rabbit antiserum (1:500; 1 hour; Jackson ImmunoResearch, USA), followed by an ABC solution (1:200, 1 hour; ABC Elite kit, Vector Laboratories, UK) and a colorimetric reaction with 3,3-diaminobenzidine tetrahydrochloride (DAB; 10 min) in 0.05M Tris-HCl buffer containing 0.003% hydrogen peroxide (Cruz et al., 2005). Sections were then washed in PBS, mounted on gelatin-coated glass slides, cleared in xylene, cover-slipped with DPX and analyzed by light microscopy.

We have a broad range of pain and inflammatory response research markers. Check us out today.