Osteoblasts Off

I am pleased to announce we now have unlabeled and FITC-labeled Osteoblasts. These are differentiated from our Umbilical Cord Blood derived Human Mesenchymal Stem Cells.

They are designed for:

• Osteogenesis/Bone Formation Studies
• Compound/Small Molecule Testing
• Gene Expression Analysis

Images: (A) Human cord-blood MSCs were expanded in low-serum MSC-Gro^TM to confluence as shown here. (B) were differentiated in osteogenic MSC-Gro^TM. Early stage osteoblasts are shown here; the arrow shows early formation of mineralized matrix. (C)&(D) Mature osteoblasts stained positive for Alizarin red. Phase contrast image at 200 x, scale bar is 50 mmeters.

I will continue to update you on new Cell Based Assay Solutions.

New Near Infrared Apoptosis Detection!

Detecting apoptosis in cells, tissue and living animals

In vitro and in vivo apoptosis detection assays are widely used in basic research and drug discovery. Neuromics has a wide range of kits providing capablilties to meet you unique requirements. We are pleased to offer even more options with our new Far Infrared Kits:
660 Polycaspase in vitro Apoptosis Detection Kit 25-50 Tests $199
660 Caspase-1  in vitro Apoptosis Detection Kit 25-50 Tests $199
NIR FLIVO™ 690 in vivo Apoptosis Tracer Kit 20 Tests-$399
NIR FLIVO™ 747 in vivo Apoptosis Tracer Kit 20 Tests-$399

Seeing is Believing

Images: To demonstrate the capabilities of the NIR-FLIVO® 747 apoptosis tracer, adult wild-type Balb/c mice were either inoculated with HSV-1 virus, which is known to induce apoptosis in the brain, or given a sham treatment. Seven days after viral inoculation, the mice were injected intravenously with either the NIR-FLIVO® 747apoptosis tracer (cat. KF17368), the NIR-FLIVO® 747 free dye (cat. KF17370, DyLight®747), or no reagent. Seven hours after reagent injection, the animals were imaged with a Carestream In-Vivo FX Pro imaging system. Strong caspase activity was located in the brain of the animal treated with HSV-1 and injected with NIR-FLIVO® 747 tracer (cat. KF17367). Minimal signal was detected in the liver region of the HSV-1-treated animal injected with the free dye control (HSV-Infected; NIR747FreeDye+) and of the uninfected mouse injected with NIR-FLIVO®747 tracer; the liver is the route of clearance for FLIVO® tracers. All reagent-injected mice show fluorescence signal in the tail where reagent is likely to pool after IV injection.

Images: Brain abscesses were induced in mice following the intracerebral inoculation of live S. aureus. Animals received intravenous injections of the pan-caspase tracer NIR-FLIVO™ 690 as an apoptosis probe or DyLight®690 (carboxylic acid form) as a control at 17 h post-infection, whereupon signals were acquired 1 h later from brain tissues immediately ex vivo using an IVIS Spectrum (Caliper Life Sciences). The same instrument settings were used to acquire both images. Strong caspase activity, detected with NIR-FLIVO™ 690 (arrows, image on right), was associated with brain abscesses, whereas minimal signal was detected in animals injected with the carboxylic acid control (image on left).

Check them all out
Apoptosis Research Reagents-Detection kits, antibodies and proteins

Everything You Wanted to Know About Stem Cells

Unforgettable way to learn about Stem Cells.

Rap on Dr. Jonathan Garlick Rap on.

If you are doing stem cell related research, check out our:

hNP1™ Human Neural Progenitor & hN2™ Neuron Discovery Kits Derived from H9 (WA09) ECSs-Consistent, Easy to Use & Cost Effective	Neural Stem Cells Media
hMPro™ Human Mesenchymal Progenitors (hMPCs) hESC Derived	Human Mesenchymal Stem Cells (hMSCs) hMSCs Derived from Umbilical Cord Blood
MSCGro™ Mesenchymal Stem Cell Media Proven and Potent Culture Growth and Differentiation Media for Mesenchymal Stem Cells (MSCs)	Cell Cryopreservation Media Maximizes cell recovery, attachment and growth
Expansion/Differentiation Kits SC to Oligodendrocytes and Dopaminergic Neurons Kits	Bioluminomics™ In Vitro Assays Brought to you by HemoGenix®
3-D Cell Based Assay Solutions Nanofibers, Hydrogels and Extracellular Matrix (ECM) Proteins	Stem Cell Research Antibodies
Stem Cell Research Proteins	Rat Neurosphere Neuroprogenitor Tissue Tissue provided live, unseparated, fresh from E18 rat cortex/hippocampus including subventricular zone.

Schwann Cells Markers

Important tool for studying diseases that cause PNS abnormalities or degeneration.

It is a goal of mine to have the best and brightest collection of Neuron-Glial Markers. Here I feature use of one of our Schwann Cell Markers. In this study our p75/NGF antibody is used as a control to determine differences in myelin sheath structure in new born pigs having the Cystic Fibrosis Mutant vs Normal Gene Expression (controls). Leah R. Reznikov, Qian Dong, Jeng-Haur Chena, Thomas O. Moninger, Jung Min Park, Yuzhou Zhang, Jianyang Du, Michael S. Hildebrand, Richard J. H. Smith, Christoph O. Randak, David A. Stoltz, and Michael J. Welsh. CFTR-deficient pigs display peripheral nervous system defects at birth. www.pnas.org/cgi/doi/10.1073/pnas.1222729110.

Images: CFTR is functionally active in Schwann cells. (A) Primary cultures of porcine Schwann cells were used 4 wk after seeding when they had developed the specific bipolar morphology and a phase-bright cell body under differential interference contrast microscopy. Schwann cells were positive for the phenotypic markers S100 and p75. (B) Whole-cell current recorded in the presence of PKA and ATP in the pipette solution and 1 min after adding 100 μM of CFTR inhibitor GlyH-101 to the bath solution. (Left) Example of currents from one cell; Inset shows voltage-pulse protocol. (Upper Right) Example of current-voltage relationship. (Lower Right) Data from five CFTR+/+ Schwann cells and seven CFTR−/− Schwann cells. *P = 0.003 (Mann–Whitney rank sum test).

Images: Fig. 1. CFTR is expressed in trigeminal nerve Schwann cells. Data are confocal microscopic images of trigeminal nerve immunostained for CFTR (green) and marker indicated above middle panels (red). Nuclei were stained with DAPI (blue). (A) Transverse cross-section with axons stained with β-tubulin III antibody. (B) CFTR−/− trigeminal nerves immunostained for CFTR and β-tubulin III.  (C) Sagittal crosssection immunostained with fluoromyelin. (D) Section stained with S100 antibody, a marker of Schwann cells. (E) Section stained with p75 antibody, also a marker of Schwann cells. (Scale bar, 20 μm.).

I will continue to post new customer pubs and other shared customer data featuring use of our Neuron-Glial Markers.