Neurotrophins-Growth Factors Update

Our Neurotrophins and Growth Factor Antibodies and Recombinant Proteins help support a wide span of research areas. These areas include: neuroscience, immunology, cardiac disease research and cancer.

I would like to update you on recent publications highlighting use of some of these reagents: Aiko Sada, Kazuteru Hasegawa, Pui Han Pin, Yumiko Saga. NANOS2 Acts Downstream of Glial Cell Line-Derived Neurotrophic Factor Signaling to Suppress Differentiation of Spermatogonial Stem Cells. DOI: 10.1002/stem.790. Copyright © 2011 AlphaMed Press...anti-GFRA1 (1:200, Neuromics, Edina, MN)...

Images: Ngn3-Cre targets GFRA1-negative cells. (A-F): At P7, Rosa-YFP; Ngn3-Cre double transgenic testes were immunostained with the indicated markers. Most of YFP-positive spermatogonia (Ngn3-lineage cells) were not stained with GFRA1. Scale bar, 100 μm. (G): Quantification of YFP-positive cells per GFRA1-positive cells at P7 and 6 weeks (N=3).

Karine Bédard, Stéphanie Segura, Stéphanie Mahaut, Catherine Tardivel, Guylaine Ferland, Bruno Lebrun, Pierrette Gaudreau. Effects of aging and caloric restriction on brainstem satiety center signals in rats. Mechanisms of Ageing and Development. dx.doi.org/10.1016/j.mad.2012.01.004...goat serum and 0.3% Triton-X-100) (Sigma–Aldrich), overnight at 4 °C with chicken anti-rat BDNF antibody (Neuromics, Edina, MN; diluted 1/200 in blocking buffer), rinsed 3× 10 min in fresh PBS and incubated for 2 h, in the dark...

Tali Kobilo, Qing-Rong Liu, Kriti Gandhi, Mohammed Mughal, Yavin Shaham and Henriette van Praag. Running is the neurogenic and neurotrophic stimulus in environmental enrichment. doi: 10.1101/lm.2283011. Learn. Mem. 2011. 18: 605-609... human recombinant BDNF (0.1 µg) monomer (Neuromics)...

Masamichi Shinoda, Masatake Asano, Daisuke Omagari, Kuniya Honda, Suzuro Hitomi, Ayano Katagiri, and Koichi Iwata. Nerve Growth Factor Contribution via Transient Receptor Potential Vanilloid 1 to Ectopic Orofacial Pain. The Journal of Neuroscience, 11 May 2011, 31(19):7145-7155; doi:10.1523/JNEUROSCI.0481-11.2011....expression immunohistochemically in TG- anti-p75 goat antibody (1:200; Neuromics)...



Images: Mandibular nerve fibers were labeled by β-NGF which was administrated into the lower lip with CFA (A) or saline (F). On day 1 after β-NGF administration into the lower lip with CFA or saline, β-NGF-positive whisker pad or lower lip TG neurons defined by FG or DiI, respectively. B, G, β-NGF-positive TG neurons. C, H, DiI-labeled TG neurons. D, I, FG-labeled TG neurons. E, J, DiI- and FG-labeled β-NGF-positive TG neurons. Mandibular nerve fibers (K) and TG neurons (L) on day 1 after labeled BSA administration into the lower lip with CFA. Open arrow, β-NGF-positive nerve fibers. Arrow, DiI-labeled β-NGF-positive TG neurons. Arrowhead, FG-labeled β-NGF-positive TG neurons. Scale bar, 50 μm. M, Frequency of β-NGF-positive neurons in FG- or DiI-labeled TG neurons after CFA or saline injection into the lower lip (n = 5 in CFA-injected group; n = 4 in saline-injected group; Student's t test).

F. R. Carreño, J. D. Walch, M. Dutta, T. P. Nedungadi, J. T. Cunningham. Brain-Derived Neurotrophic Factor-Tyrosine Kinase B Pathway Mediates NMDA Receptor NR2B Subunit Phosphorylation in the Supraoptic Nuclei Following Progressive Dehydration. Journal of Neuroendocrinology. Volume 23, Issue 10, pages 894–905, October 2011. DOI: 10.1111/j.1365-2826.2011.02209.x...TrkB receptor (Neuromics, Edina, MN, USA)...

Jie Shen, Yoko Ishii, Guihua Xu, Thanh Chung Dang, Takeru Hamashima, Takako Matsushima, Seiji Yamamoto, Yuichi Hattori, Yusuke Takatsuru, Junichi Nabekura and Masakiyo Sasahara. PDGFR-β as a positive regulator of tissue repair in a mouse model of focal cerebral ischemia. Journal of Cerebral Blood Flow & Metabolism , (28 September 2011). doi:10.1038/jcbfm.2011.136...goat polyclonal anti-PDGFR-β antibody (1:100; Neuromics, Edina)...

Thomas Karsten Kilvaer, Andrej Valkov, Sveinung W. Sorbye, Tom Donnem, Eivind Smeland, Roy Martin Bremnes,and Lill-Tove Busund. Platelet-Derived Growth Factors in Non-GIST Soft-Tissue Sarcomas Identify a Subgroup of Patients with Wide Resection Margins and Poor Disease-Specific Survival. Volume 2010 (2010), Article ID 751304, 10 pages doi:10.1155/2010/751304...PDGF-CC (goat polyclonal; GT15151; Neuromics; 1 : 80)...

I will keep you posted on new developments.

Enhancing Neurite Outgrowth

We are pleased to announce the addition of new proteins to our Axon Growth and Guidance category. These are designed to enhance neurite out growth.

Name	Catalog #	Type	Species	Size	Price
NGF-b, NSO Derived	PR15083	Protein	H; M; R	100 ug	$285
NGF-b, NSO Derived, CF	PR15084CF	Protein	H; M; R	100 ug	$285
S100A13, CF	PR15085CF-50	Protein	H; R	50 ug	$315
Slit1	PR15075-50	Protein	Ch; H	50 ug	$315
Slit2, CHO	PR15085-50	Protein	Ch; H	50 ug	$315

Image: Cultured chick dorsal root ganglion neurons were grown in the presence of recombinant human NGF-b with (A) or without (B) recombinant mouse Slit2. The presence of the Slit2 protein signifi cantly enhanced neurite outgrowth.