Immune Response Research Solutions

Cytokine Markers and More
Immune and inflammatory response are elevated in most diseases. We have strong solutions for detecting these responses. These include cytokine markers and panels.

Here's a new pub referencing use of one of our Interleukin Markers. ArdoSabir and AndiSumidarti. Interleukin-6 expression on inflammed rat dental pulp tissue after capped with Trigona sp. propolis. Saudi Journal of Biological Sciences Volume 24, Issue 5, July 2017, Pages 1034-1037. doi.org/10.1016/j.sjbs.2016.12.019 ...Four rats were sacrificed at 6 h, 2 days, 4 days and 7 days respectively. The teeth and surrounding bone were resected, fixed in Bouin’s fixative for 24 h, decalcified with acetic acid/formal saline for 7 days, embedded in paraffin and sectioned serially at 6 μm thickness. The sections were stained with IL-6 monoclonal antibody (Neuromics, USA) using immunohistochemistry method and viewed by light microscopy...

Image: Interleukin-6 expression on rats dental pulp tissue. Normal dental pulp with no treatment (negative control) in groups I (A–B) and inflamed dental pulp in groups II (C–D), III (E–F), IV (G–H) and V (I–J), capped with Ethanolic Extract Propolis (EEP), Extract Flavonoid-Propolis (EFP), Extract Non-Flavonoid Propolis (ENFP), and Calcium Hydroxide (Ca(OH)2), respectively. Arrows show IL-6 expression. Immunohistochemistry method, DAB chromogen, original magnification 200×.

Proven and Published Sandwich ELISA Kits

Growing Catalog of ELISA Kits

We are partnering with RayBiotech to manufacture our ELISA Kits. They have proven valuable additions to our Cell Based Assay solutions.

Neuromics has been built on a foundation of quality. RayBiotech's commitment to quality includes compliance to GLP/GMP FDA and ISO9000 regulations.

ELISA Kits Sample Data

Figure: Induction of proinflammatory cytokines is attenuated in CX3CL1−/− mice expressing sFKN. TNFα and IL-1β concentrations were measured using standard ELISA techniques for VM lysates. a, TNFα concentrations were upregulated following MPTP administration (three-way ANOVA; F(1, 23) = 18.36, ★★★p < 0.001). Comparatively, CX3CL1−/− mice expressing sFKN in the SNpc had significantly lower concentrations of TNFα relative to mFKN (Tukey's HSD; ***p < 0.001) and GFP (Tukey's HSD; ###p < 0.001) expressing mice. There were no significant differences between sFKN and WT-MPTP (Tukey's HSD; p = 0.384) or mFKN and GFP (Tukey's HSD; p = 0.773). b, The IL-1β concentrations in the VM were significantly upregulated for mice exposed to MPTP (three-way ANOVA; F(1, 23) = 11.97, ★★★p = 0.002). Similar to the pattern of TNFα, IL-1β concentrations in CX3CL1−/− mice expressing sFKN were significantly blunted compared to both mFKN (Tukey's HSD; ***p = 0.001) and GFP (Tukey's HSD; ###p < 0.001). As was observed with TNFα, there were no significant differences in IL-1β concentrations between WT-MPTP and sFKN (Tukey's HSD; p = 0.785) or mFKN and GFP (Tukey's HSD; p = 0.845) expressing mice. Data are presented as the mean ± SEM.

Questions? Please contact me directly, Pete Shuster, CEO and Owner, 612-801-1007 or pshuster@neuromics.com. Thank you.

Cytokines and Neuropathic Pain

I have been frequently posting results of testing blood serum of our clients suffering from Autoimmune (including Neuroimmuno) Diseases and Autistic Children. Our testing includes both standard medical testing (serum levels of toxins, metals, pathogen loads and markers like Cortisol, CRP, CDs, ALPS, NSE, S100b). In addition, we also doing testing using our Quantibody® Antibody.

Many of the clients report chronic mild to severe neuropathic pain.

By definition, this pain encompasses a series of heterogeneous conditions with some similar clinical manifestations. Peripheral examples include traumatic nerve injury, diabetic peripheral neuropathy and chemotherapy-induced peripheral neuropathy or Multiple Sclerosis. (see: Front Pharmacol. 2013; 4: 142.Published online Nov 22, 2013. doi: 10.3389/fphar.2013.00142). It is hard to unravel the cause and effects of this pain,

However, in analyzing the results from our clients' test we notice a relationship between dysregulation of some key cytokines and levels of pain (more data is required to confirm this). Here're our results (n=5)

Figure: Clients reporting pain vs healthy controls.

We will continue posting results here. We are releasing a Custom Quantibody Neuroimmuno-Pain Array which will include more bio-markers suspected to play a role in pain signaling.

Antibody Array-ELISA Kits for Cancer Researchers

We continue to build our catalog of Antibody Arrays & ELISA Kits
for Cancer Researchers. Our RayBio® C-Series Ctyokine Arrays
enable you to analyze the expression of up to 120 Cytokines for about
the price of a single ELISA (585 USD).

 
Figure 3. PTEN Downregulation in HER2-Overexpressing Cells Activates an IL6/NF-kB-Mediated Inflammatory Feedback Loop (A–E) MCF7-HER2+PTEN cells secreted 3- to 5-fold higher levels of IL6, IL8, and CCL5 compared to MCF7-HER2+ or MCF7-PTEN cells as determined by RayBio human cytokine antibody Array 5 (A). The intensity of each blot compared to control was determined by Kodak image analyzer (B) and confirmed by ELISA (C). Downregulation of PTEN in HER2-amplified breast cancer cells, BT474, SKBR3, HCC1954, and Sum159-HER2+ (D) results in increased levels of these cytokines in vitro (E). http://dx.doi.org/10.1016/j.molcel.2012.06.014

Array Capabilities:

• Detects expression of 96 secreted proteins in a single sample, in a single day. 
• If you can do a Western, you can use a C-Series Antibody Array. And get excellent results the first time!  
• Sandwich ELISA pairs give C-Series arrays high sensitivity and specificity.  
• C-Series arrays are compatible with practically ANY liquid sample. 

References have cited using membrane-based C-Series Cytokine Antibody Arrays with nearly every liquid sample type imaginable, including: cell-cultured and co cell cultured media, cell and tissue lysates, tissue/organ perfusates, serum, plasma, urine, and many other body fluids, including cyst, interstitial, synovial, blister, cerebrospinal, prostatic and amniotic fluids, as well as abscesses, broncho-alveolar lavage, sputum, saliva, tears, breath condensates, and even human milk and colostrum. Publications referencing use of our Antibody Arrays.

I will be posting more new developments here.