Neuromics Human Cells at Work

Work Great in 3-D Assays

Neuromics Human Primary Cells are being used with increasing frequency in 3-D Cell-Based Assays. Last month, we posted a publication referencing use of our Human Pericytes in a Blood-Brain Barrier Model.

In this publication, our GFP-Labeled HUVECS are used in a 3-D Microfluidics Chip Model to study the impact of indoor airborne particles on human health. Yan Li, Chuanlin Hu, Pengcheng Wang, Yan Liu, Luyang Wang, Qingmeng Pi, Zhiyong Gong, Xu Yang, Michael Mak and Yang Wu (2019). Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip. Journal of Nanobiotechnology 2019 17:20. https://doi.org/10.1186/s12951-019-0458-2

Images: Development progress of human umbilical vein endothelial cells (HUVECs) by 2D culture and 3D culture. a 2D HUVEC culture in a disk, b–e camera image of fluorescent HUVECs developed from day 1 to day 4. f 3D HUVEC culture in a chip, g–j camera image of fluorescent HUVECs developed from day 1 to day 4.

Remember, we have a 100% money back policy should the cells not work in your hands.

Human Primary Cells Trifecta

Tri-culture of Neuromics' Human Primary Cells in 3-D BBB Model

I mentioned in an earlier post that we take Researchers questioning the validity of our Human Primary Cells very seriously. We follow up with our clients to make sure our cells are working as expected. If they are not 100% happy, we offer a free replacement or full refund.

...So it is encouraging to see Researchers using our Human Pericytes,  Brain Microvascular Endothelial Cells (HBMES) and Astrocytes to build a static 3-D Blood-Brain Barrier Model.

Ece Bayir, M. Mert Celtikoglu, Aylin Sendemira. The use of bacterial cellulose as a basement membrane improves the plausibility of the static in vitro blood-brain barrier model. https://doi.org/10.1016/j.ijbiomac.2018.12.257.

Image: Pericytes, HBMECS, and Astrocytes 5 days after culturing. Live cells (green) and dead cells (red).

The investigators concluded, "Caffeine and sucrose permeability values obtained from all models were close to literature data and physiological values."

Primary Human Brain Pericytes

Pure and Potent
The authors of a chapter on pericytes published in SpringerLink referred to our human primary pericytes as "pericyte-like", but chose not to characterize them because of the "exorbitant cost". Dore-Duffy P., Esen N. (2018) The Microvascular Pericyte: Approaches to Isolation, Characterization, and Cultivation. In: Birbrair A. (eds) Pericyte Biology - Novel Concepts. Advances in Experimental Medicine and Biology, vol 1109. Springer, Cham

Given that these pericytes are also part of our hot selling 3-D Human BBB Model, we are in the process of challenging these claims. We will always take inaccurate claims regarding our solutions seriously, and we take immediate action.

• Characterization of cells-we validated several key markers by immunofluorescence.

Staining of Desmin (dilution 1:100). Secondary antibody conjugated to Alexa 594 (red) and counterstained with DAPI (blue). Cells were mounted using iBrite mounting media.

Staining of Actin (dilution 25 ug/mL). Secondary antibody conjugated to Alexa 594 (red) and counterstained with DAPI (blue). Cells were mounted using iBrite mounting media.

We also plan on doing a phenotypic analysis of these cells and will post here when completed.

• Cost of cells-isolating pericytes from human donors, and expanding to the required number of cells involves much time and effort. We know! Further differentiating stem cells into pericytes is hard and time-consuming. Against this backdrop, we consider our pricing to be inexpensive. Though they are more difficult to derive, they are priced equivalently with our other human primary cells ($789/500,000 USD Cells).

As always, we will post new data here.