Altered expression of circular RNA (circRNA) is recognized as a contributor to malignant pain where microRNA (miRNA) exerts an essential effect. Researchers used our i-FectTMTransfection Kit to knock them down. Zhongqi Zhang, Xiaoxia Zhang, Yanjing Zhang, Jiyuan Li, Zumin Xing. Yiwen Zhang. Spinal circRNA-9119 Suppresses Nociception by Mediating the miR-26a-TLR3 Axis in a Bone Cancer Pain Mouse Model. Spinal circRNA-9119 Suppresses Nociception by Mediating the miR-26a-TLR3 Axis in a Bone Cancer Pain Mouse Model. Journal of Molecular Neuroscience. pp 1–10
Intrathecal Administration of miRNA and circRNA Pre-miRNA sequence of miR-26a and circ9119 were cloned into a plasmid. The i-Fect transfection reagent (10 μL; Neuromics, Edina, USA) was used to resuspend plasmids for injection.
Sample Data
Image: siRNA-mediated suppression of target gene expression in Schwann cells.
i-Fect Kits sell for 399 USD. They are widely used and frequently published. Check the out today.
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