Human Pancreatic CAFS and Tumor Dynamics

 Extracellular Matrix (ECM) Matters
Our Human Pancreatic Cancer Fibroblasts were recently featured in a publication focusing on the role of the ECM on tumor dynamics. Andreas Stylianou, Vasiliki Gkretsi, Maria Louca, Lefteris C. Zacharia, and Triantafyllos Stylianopoulos (2019). Collagen content and extracellular matrix cause cytoskeletal remodelling in pancreatic fibroblasts. J. R. Soc. Interface 16: 20190226. http://dx.doi.org/10.1098/rsif.2019.0226.

Commercially available pancreatic native human FBs and,CAFs (cat. nos. SC00A5 and CAF08, respectively, Neuromics) were cultured in MSC-GRO (VitroPlus III, low serum, complete,
cat. no. SC00B1, Neuromics) medium in a 5% CO2-incubator at 37^OC.

Figure: Representative CAFs spheroids embedded in collagen gels at time 0 and at 6 h post implantation, respectively.

The results demonstrated that collagen concentration and consequently ECM stiffening differentially modulates FBs and CAFs cytoskeleton remodelling and invasion with stress fibre orientation being significantly altered in FBs. Finally, the presence of TGF-b reverses the suppressive effect of collagen stiffness on cell invasion.

Neurofilament Antibodies-Check Them Out

Widely Used and Frequently Published
Our Neurofilament Antibodies are well characterized and research ready. Here is a recent publication using one of our Neurofilament Heavy Antibodies. Jennifer M. Hahn, Kelly A. Combs, Christopher M. Lloyd, Kevin L. McFarland, Steven T. Boyce, and Dorothy M. Supp. Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting to full thickness wounds. PLoS One. 2019; 14(3): e0213325. Published online 2019 Mar 5. doi: 10.1371/journal.pone.0213325.

Figures: Merkel cells in grafted ESS are associated with neurons expression neurofilament heavy (NF-M) by eight weeks after grafting. Immunochemistry with antibodies against NF-H (red) and KRT20 (green) was used to localize neurons and Merkel cells, respectively, in ESS after grafting to mice. Nuclei were counterstained with DAPI (blue; B, D, F, H). Shown are cross sections of ESS at 4 weeks (A-B), 8 weeks (C-D), 12 weeks (E-F), and 14 weeks (G-H) after grafting; each row contains images of the same section. Scale bars in A is same for all images. White arrows indicate examples of NF-H-positive nerves associated with or in proximity to Merkel cells; yellow arrows indicate NF-H-positive nerves not associated with Merkel cells.

Remember. All our antibodies come with a money back guarantee. Your satisfaction is joh #1 for us. 

We Can Sell and Deliver FBS in Large Volumes

This is Includes Single Lots
We are pleased with the growth in our FBS business. This is being made possible, in part, to our ability to find the "sweet spot" by coupling low pricing with high quality.

We offer all potential customers free samples to test with the idea that they find the "sweet spot." This leads to happy customers and repeat business.

Here's a volume shipment wrapped and ready to go.

2 pallets of 700X500 ml. Bottles FBS.

Need FBS? Just ask us. Learn more by emailing rose@neuromics.com. Thank you!

i-Fect Scores Again

Knock-down of HIF-1a Attenuates Chemo Induced Pain
i-Fect ^TM is one of our original products. It has enjoyed 14 years of upping transfection percentages both in-vitro and in-vivo.

Here is a new study showing successful use of i-Fect to knock down HIF-1a in-vivo-Taylor Ludman and Ohannes K. Melemedjian. (2019). Bortezomib-induced aerobic glycolysis contributes to chemotherapy-induced painful peripheral neuropathy. Molecular Pain. https://doi.org/10.1177/1744806919837429.

Figure 1. (a) Treatment of mice with bortezomib (Bor) for five days augmented HIF1A expression in L4-6 DRGs (*P ¼ 0.0412, five mice/ group) relative to the vehicle-treated group. (b) A schematic depicting the site of the intrathecal (IT) siRNA injection. The siRNA was administered between the L4 and L5 vertebrae which is around 17 mm rostral to the spinal cord (SC) section innervated by the L4-6 DRGs. (c) IT injection of siRNA (1 mg in 5 ml) that targets HIF1A (siRNA) but not control siRNA (Cont), for two consecutive days, significantly reduced the levels of HIF1A in L4-6 DRGs. (***P=0.0006, five mice/group). (d) IT siRNA did not affect HIF1A levels in L4-6 spinal cord (five mice/group). (e) After determining baseline withdrawal thresholds using von Frey filaments, male ICR mice received IP injection of vehicle or bortezomib (black arrows) and IT siRNA (blue arrows). The withdrawal thresholds were measured on days 7 to 14. IT HIF1A siRNA prevented the development of bortezomib-induced neuropathic pain. (****P less than 0.0001, five mice/group). DRG: dorsal rootganglia; HIF1A: hypoxia-inducible factor 1 alpha; IT: intrathecal; IP: intraperitoneal; siRNA: small interfering RNA.

This study is the first to demonstrate that the stabilization of HIF1A expression underpins the development of bortezomib-induced neuropathic pain. Crucially, these findings reveal that the initiation and maintenance of bortezomib-induced neuropathic pain are regulated by distinct mechanisms.