We are pleased to announce the addition of Medical Grade Collagen to our Cell Based Assay Solutions. Applications include:
- Tissue engineering
- Wound healing
- Medical device coatings
- 3D cell cultures
- Drug delivery
- Sealants
- Electrospinning
- Hemostats
- 3D printing
This example outlines how this collagen can be used for cartilage regeneration from mesenschymal stem cells. Acta Biomater. 2016 Jan;30:212-221. doi: 10.1016/j.actbio.2015.11.024. Epub 2015 Nov 18.
Figure: Fabrication of macroporous woven scaffolds and pellet delivery via the macroporous woven collagen scaffold; (a) Liquid to solid phase transition of collagen molecules via electrocompaction to fabricate electrochemically aligned collagen threads and electrocompacted sheets. (b) Collagen thread is woven around a set of pins and threads are stabilized by crosslinking two collagen sheets on top and bottom of woven part of scaffold. (c) Schema of the final woven collagen scaffold. (d) 1 million MSCs pelletized at 500 ×g for 12 minutes, cultured for 3 days and then transferred in to scaffold holes.
SIGNIFICANCE: Mesenchymal condensation is critical for driving chondrogenesis, making high density cell seeding a standard in cartilage tissue engineering. Efforts to date have utilized scaffold free delivery of MSCs in pellet form. This study developed a macroporous scaffold that is fabricated by weaving highly aligned collagen threads. The scaffold can deliver high density cell condensates while providing mechanical stiffness comparable to that of cartilage. The scaffold also mimicked the arcade-like orientation of collagen fibers in cartilage. A highly robust chondrogenesis was observed in this mesenchymal cell pellet delivery system. Baseline mechanical robustness of this scaffold system will enable delivery of cell pellets as early as three days.
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