Studying Apoptosis

By Cancer Researchers

Our Apoptosis Kits have proven rock solid in the hands of cancer researchers.

Here's data from a study of the impact of Vestibular schwannoma (VS) on hearing loss.

Images: Spiral Ganglion Cells labeled with our polycaspase kit stain to identify apoptotic cells.

Culturing Neurons on Conductive Biomaterials

Neuromics' Neurons on Graphene
Conductive biomaterials are an ideal bio-substrate for modifying cellular behaviors by conducting either internal or external electrical signals.

In this study, researchers successfully culture our cortical neurons on Nonfunctionalized graphene nanosheets (NGN): Shiyun Meng, Rong Peng. Growth and Follow-Up of Primary Cortical Neuron Cells on Nonfunctionalized Graphene Nanosheet Film. Article first published online: January 18, 2018. https://doi.org/10.5301/jabfm.5000263.

Figure: Spray-coating a nonfunctionalized graphene nanosheet (NGN) on a glass slide. To fix the NGN onto the glass slide, polyurethane (PU) was firstly spin-coated onto glass slides as a polymer matrix, then NGN particles were spray-coated on by airbrush.

Figure: Nuclei formed in 7 and 14 days of cell culture are shown at a relatively high magnification (scale bar = 50 μm): 1/500 MAP-2 (H-300) and 1/500 Alexa Fluor® 488 anti-Rabbit stained green for neuron microtubules and DAPI stained blue neuron nuclei.

We have a large catalog of potent, proven and pure human and animal cells.

Cells from Diseased Donors

Focus on Neuro Diseases

We now have the capability to provide cells of the central and peripheral nervous system from donors with Neuro diseases. These include cells from donors with ALS, AD, PD, and Brain Cancer Donors, to name a few.

We have provided cells to virtually all the large Pharmas and many small and mid-size Bio-techs. We have worked with Novartis to gain 21-CFR compliance for cells that they are using for their eye diseases drug discovery programs.

I am at your “beck and call” should you have interested in exploring specific capabilities further. You can e-mail or call me at 612-801-1007

Microvascular Endothelial Cells

Tested, Characterized and Research Ready
Our Microvascular Endothelial Cells continue to work and work in the hands of our customers.

Check out these pubs:
1. Odunayo O. Mugisho, Colin R. Green, Jie Zhang, Nicolette Binz, Monica L. Acosta, Elizabeth Rakoczy and Ilva D. Rupentha. (2017). Immunohistochemical Characterization of Connexin43 Expression in a Mouse Model of Diabetic Retinopathy and in Human Donor Retinas. Int. J. Mol. Sci. doi: 10.3390/ijms18122567
 2. Michael Anthony Ruiz, Biao Feng, and Subrata Chakrabarti. (2015). Polycomb Repressive Complex 2 Regulates MiR-200b in Retinal Endothelial Cells: Potential Relevance in Diabetic Retinopathy. PLoS One.10(4): e0123987. doi: 10.1371/journal.pone.0123987.

In these, our cells are used as "healthy controls" to study Diabetic Retinopathy.

Figure: Connexin43 (green) and GFAP (red) expression in normal and human DR donor retinas in regions of extensive vascular damage. Large cells (white arrows, left column) represent non-specific auto-fluorescent amacrine cells. Connexin43 expression was markedly higher in the GCL of DR donor tissues compared to age-matched controls, and was strongly expressed throughout all retinal layers. GFAP labeling was also markedly higher in DR compared to normal donor eyes representing hyper-reactive Müller cells. Connexin43 expression was increased in regions identified as blood vessels and correlated with increased GFAP labeling at these sites, indicating glial cell activation (white circle). GCL = ganglion cell layer; IPL = inner plexiform layer; INL = inner nuclear layer; OPL = outer plexiform layer; ONL = outer nuclear layer. Scale bar: 200 µm

We stand ready to serve you. Pete Shuster, CEO and Owner, pshuster@neuromics.com

Medical Grade Soluble Collagen

New Products-New Applications

We are pleased to announce the addition of  Medical Grade Collagen to our Cell Based Assay Solutions. Applications include:

• Tissue engineering 
• Wound healing
• Medical device coatings
• 3D cell cultures
• Drug delivery 
• Sealants 
• Electrospinning
• Hemostats
• 3D printing

This example outlines how this collagen can be used for cartilage regeneration from mesenschymal stem cells. Acta Biomater. 2016 Jan;30:212-221. doi: 10.1016/j.actbio.2015.11.024. Epub 2015 Nov 18.

Figure: Fabrication of macroporous woven scaffolds and pellet delivery via the macroporous woven collagen scaffold; (a) Liquid to solid phase transition of collagen molecules via electrocompaction to fabricate electrochemically aligned collagen threads and electrocompacted sheets. (b) Collagen thread is woven around a set of pins and threads are stabilized by crosslinking two collagen sheets on top and bottom of woven part of scaffold. (c) Schema of the final woven collagen scaffold. (d) 1 million MSCs pelletized at 500 ×g for 12 minutes, cultured for 3 days and then transferred in to scaffold holes.

SIGNIFICANCE: Mesenchymal condensation is critical for driving chondrogenesis, making high density cell seeding a standard in cartilage tissue engineering. Efforts to date have utilized scaffold free delivery of MSCs in pellet form. This study developed a macroporous scaffold that is fabricated by weaving highly aligned collagen threads. The scaffold can deliver high density cell condensates while providing mechanical stiffness comparable to that of cartilage. The scaffold also mimicked the arcade-like orientation of collagen fibers in cartilage. A highly robust chondrogenesis was observed in this mesenchymal cell pellet delivery system. Baseline mechanical robustness of this scaffold system will enable delivery of cell pellets as early as three days.