I wanted to share some of the tips and data shared during the ArunA Biomedical's/Axion Biosystem's Webinar on MEA and our Mouse Motor Neurons
Plating Cells on Axion's MEA
- Surface Coating PEI-laminin for adhesion and uniform monolayer development
- Dotting Constrains cells to the array
- Requires fewer cells per well
- Media changes every 2-3 days
- Variations on Cell Density
Different Plating Densities
At these densities there was the least variation in mean firing rates. This data shows as the density increased the cells moved towards firing in synchronicity.
The demand for using these motor neurons for neuromuscular diseases drug discovery has been brisk and growing. Should you have question on how they would work for your unique applications, do not hesitate to contact me directly @ 612-801-1007 or pshuster@neuromics.com. Pete Shuster, CEO and Owner, Neuromics
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