Healthy and Happy Neuron/Astrocytes Cultures

A Track Record of Customer Success

Neuromics is  recognized for the quality of  hNP1™ Human Neural Progenitor,  hN2™ Neuron Discovery Kits, E18 and E20 Rat Primary Neurons and E18 Rat Primary Astroglia. As the company owner, it is important that I keep my finger on the pulse of how well they work for each and every unique application. I personally follow up with each user and if there are any issues, we replace the cells once free of charge. Your success is critical to our growth.

I wanted to share with you recent references. These give and excellent snapshot of the exciting ways our cells can used. Alexzander Asea, Punit Kaur, Alexander Panossian, Karl Georg Wikman, Evaluation of molecular chaperons Hsp72 and neuropeptide Y as characteristic markers of adaptogenic activity of plant extracts. Phytomedicine, Available online 6 August 2013, ISSN 0944-7113, http://dx.doi.org/10.1016/j.phymed.2013.07.001
...using trypan blue exclusion test and routinely found to contain less than ;5% dead cells. Primary human neurons were purchased from Neuromics (Edina, MN)...

Images: Micropictograph of primary culture from micro-dissected hippocampus. (A) Neurons are round and healthy 1 h after plating on poly-d-lysine substrate. (B) Five days in culture, neurons remain healthy and have extended processes. Magnification 60×. http://dx.doi.org/10.1016/j.phymed.2013.07.001

Todd GK, Boosalis CA, Burzycki AA, Steinman MQ, Hester LD, et al. (2013) Towards Neuronal Organoids: A Method for Long-Term Culturing of High-Density Hippocampal Neurons. PLoS ONE 8(4): e58996. doi:10.1371/journal.pone.0058996
... a protocol that allows for culturing of E18 hippocampal neurons at high densities for more than 120 days. These cultured hippocampal neurons are (i) well differentiated with high numbers of synapses, (ii) anchored securely to their substrate, (iii) have high levels of functional connectivity, and (iv) form dense multi-layered cellular networks. We propose that our culture methodology is likely to be effective for multiple neuronal subtypes–particularly those that can be grown in Neurobasal/B27 media. This methodology presents new avenues for long-term functional studies in neurons...
Xiugong Gao, Hsiuling Lin, Radharaman Ray, Prabhati Ray. Toxicogenomic Studies of Human Neural Cells Following Exposure to Organophosphorus Chemical Warfare Nerve Agent VX. Neurochemical Research. February 2013.
...Human hN2 neurons were obtained from Neuromics...

Image: Staining of hN2 Human Neurons with Tuj 1 (Neuron-specific class III beta-tubulin) (red) and Nestin (green). Counter stained with DAPI (blue). hN2 Cells-Electro Phys Data. 

Xiufang Guo, Severo Spradling, Maria Stancescu, Stephen Lambert, James J. Hickman. Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1. Biomaterials, In Press, Corrected Proof,Mar 2013.doi:10.1016/j.biomaterials.2013.02.061
...hNP1, were obtained from Neuromics (Edina, Minnesota)...
Wei Zhang , Radhia Benmohamed, Anthony C. Arvanites, Richard I. Morimoto, Robert J. Ferrante, Donald R. Kirsch, Richard B. Silverman. Cyclohexane 1,3-diones and their inhibition of mutant SOD1-dependent protein aggregation and toxicity in PC12 cells. Bioorganic & Medicinal Chemistry. Elsevier Ltd. All rights reserved.doi:10.1016/j.bmc.2011.11.039.
...Primary rat cortical tissue was purchased from Neuromics Inc., Edina, MN and used to initiate primary cortical neuron cultures. The tissue was isolated from micro-surgically dissected E18 embryonic Sprague/Dawley or Fischer 344 rat brain and shipped in a nutrient rich medium under refrigeration. To isolate neurons, the tissue was incubated with papain at a concentration of 2 mg/mL in Hibernate without calcium for 30 min at 37^OC. The enzymatic solution was then removed, and 1 mL of culture media (Neurobasal, B27, 0.5 mM glutamine) was added. A sterile Pasteur pipette was used to gently disperse the cells, which were then washed, re-suspended and counted. The cells were plated on poly-D-lysine coated 96-well plates at a density of 20,000 cells/well and incubated at 37^OC in a 5% CO2-humidified atmosphere for 5 days prior to use in compound testing. By microscopic inspection, the resulting cultures consisted of app. 90% neurons...
Majumder A, Dhara SK, Swetenburg R, Mithani M, Cao K, Medrzycki M, Fan Y, Stice SL. Inhibition of DNA methyltransferases and histone deacetylases induces astrocytic differentiation of neural progenitors. Stem Cell Res. 2013 Jul;11(1):574-86. doi: 10.1016/j.scr.2013.03.003. Epub 2013 Apr 2.
...Progenitor to Astrocytes Protocol: For astrocytic differentiation of hNP cells, neuronal differentiation media were supplemented with BMP2 (20 ng/mL) and combinations of Aza-C and TSA; Aza-C (500 nM), TSA (100 nM) and BMP2 (20 ng/mL) for 2 days, with one complete media change in between, followed by differentiation media supplemented with BMP2 but not with Aza-C or TSA. Cells were harvested prior to analysis at 5, 15 or 30 days of treatment or for cryopreservation at d6 or d10 of differentiation. For cryopreservation, cells were dissociated with Accutase™ and frozen in differentiation media containing10% DMSO. Viability was assessed at 30 days in Aza-C and TSA treated cultures by trypan blue exclusion, and datawas acquired using a Cellometer Auto T4® (Nexcelom Biosciences)...
Aparna Talekar, Antonello Pessi, and Matteo Porotto. Infection of primary neurons mediated by Nipah virus envelope proteins: Role of host target cells in antiviral action. J. Virol. doi:10.1128/JVI.00452-11.
...Hippocampus, Cortex and Ventricular Cells (Neuromics)...

I will continue posting results here.