Striatal Neurons Image

Thank you to Dr. Henry Guo, Intracellular Therapies

Larger Striatal Image (10X)

E18 and E20 Rat Primary Neuronal Tissue -NEURON CULTURES
Fresh Tissue and media are provided to initiate a culture of primary neurons.
E18 Rat Primary Neuronal Tissue - ASTROCYTE CULTURES
Fresh Tissue and media are provided to initiate a culture of primary astrocytes.
Human Brain Tissue (Blocks, Lysates and Slides)-New
Healthy adult human brain tissue
Frozen Primary Rat Neurons-NEW
From E18 Rat Cortex
Frozen Primary Mouse Neurons-New
Growth Media and Poly-D-Lysine Coverslips

SfN Presentation-i-Fect and siRNA in vivo

We are excited about this upcoming presentation at Society for Neuroscience comference presesentation. Neuromics' i-Fect ™ reagent was used to deliver siRNA in vivo to silence the NTS2 gene.

Program#/Poster#:509.6/PP9
Title:Small interfering RNA-mediated selective knockdown of NTS2 receptors reverses neurotensin-induced analgesia in rats
Location:San Diego Convention Center: Halls B-H
Presentation Start/End Time:Monday, Nov 05, 2007, 2:00 PM - 3:00 PM
Authors:*L. DORE-SAVARD1,2, G. ROUSSY1, M.-A. DANSEREAU1, K. BELLEVILLE1, N. BEAUDET1, M. BEHLKE2, P. SARRET1; 1
Physiology and Biophysics, Univ. Sherbrooke, Sherbrooke, PQ, Canada; 2Integrated DNA Technologies Inc., Coralville, IA

2007 Copyright by the Society for Neuroscience all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.

Musashi Customer Data

Musashi-1

Image: Musashi-positive cells in the ventricular zone of E14.5 mouse medulla. Picture was taken at 200x magnification. The sections are 4% PFA fixed, paraffin-embedded and cut at 5 micron. Courtesy of Xi Huang, Chiang Lab, VUMC

Hot off the Presses

Blackwell Synergy - Headache, Volume 47 Issue 7 Page 1008-1023 ...07 Jun 2007 Issue online: 12 Jul 2007. Accepted for publication April 19, .... Neuromics, Edina, MN, USA), active p38 MAP kinase (1:200; Cell Signaling ...
www.blackwell-synergy.com/doi/abs/10.1111/j.1526-4610.2007.00854.x - Similar pages

New GF Proteins

We are pleased to inform you of new Growth Factor Proteins. These reagents are well characterized, research proven and data rich. Even better, they are priced at a level that makes the intial buy decision easy. These are the initial proteins we have added to our catalog. We will be adding 30-50 per month and keep you updated.

BMP-7 CHO
CNTF
EGF
EGF
EGF Pichia
EGF-21 Leu
FGF-1, acidic
FGF-1, acidic, Sf9
FGF-19
FGF-2
FGF-2, basic
FGF-2, basic, Sf9
FGF-21
FGF-22
FGF-9
FGF-9
GDNF
NGF-b
NGF-b CHO
ProNGF

Image: GDNF Structure and Sequence

Nesting Staining of Grafted Human Neural Progenitor Cells

We would like to thank Dr. Rike Zietlow of Cardiff University for generously providing us this image.

Image: Nestin (Catalog# GT15012) staining of human neural progenitor cells grafted into the rat brain, 24 weeks post-op. One is of processes running along the host corpus callosum, one of cells in the hippocampus, weakly counterstained with human nuclear antigen (brown). Nestin concentration was 1:1000, overnight at room temp, visualised using the DAKO vector SG kit.

APJ (Apelin Receptor) in the Gut

Great Image of our APJ-Catalog#:MO15011

Image: Confocal microscopy of rabbit gastric gland distinguishing the distribution of HDC and the apelin receptor. Green pseudocolor: FITC-coupled polyclonal anti-HDC ab. Red pseudocolor: TRITC-coupled monoclonal anti-APJ receptor ab. Two cells in this gland show specific anti-AJP receptor and anti-HDC staining. Right: a higher magnification scan of the cell in the top right corner of the left panel showing peripheral APJ receptor localization and central perinuclear HDC staining. Physiol. Genomics 25: 153-165, 2006

APJ (Apelin Receptor) in the Gut

Great Image of our APJ-Catalog#:MO15011

Image: Confocal microscopy of rabbit gastric gland distinguishing the distribution of HDC and the apelin receptor. Green pseudocolor: FITC-coupled polyclonal anti-HDC ab. Red pseudocolor: TRITC-coupled monoclonal anti-APJ receptor ab. Two cells in this gland show specific anti-AJP receptor and anti-HDC staining. Right: a higher magnification scan of the cell in the top right corner of the left panel showing peripheral APJ receptor localization and central perinuclear HDC staining. Physiol. Genomics 25: 153-165, 2006

Ret in Recent Pub

Our Ret-Catolog#: GT15002 is referenced in the publication:
Bhupinder P.S. Vohra , William Planer , Jennifer Armon , Ming Fu , Sanjay Jain , Robert O. Heuckeroth Reduced endothelin converting enzyme-1 and endothelin-3 mRNA in the developing bowel of male mice may increase expressivity and penetrance of Hirschsprung disease-like distal intestinal aganglionosis
Developmental Dynamics 236:106–117, 2007... goat anti-Ret antibody (Neuromics,. Inc., Minneapolis, MN, 1:200)...























Figure 2. Testosterone and Mullerian inhibiting substance (MIS) do not have deleterious effects on enteric nervous system (ENS) precursor proliferation, differentiation, or survival. A-D: Cultured immunoselected embryonic day (E) 12.5 C57BL/6 ENS precursors were grown for 24 hr with or without glial cell line-derived neurotrophic factor (GDNF), testosterone, or MIS. A-C: Triple-label immunohistochemistry was performed with antibodies to Ret (A), neuron-specific beta III tubulin (TuJ1; B), or histone 3 phosphate (H3P; C). D: Shows the merged image. Arrowheads show a Ret+ TuJ1+ H3P- cell. Arrows identify a cell with a punctate nuclear staining pattern characteristic of H3P immunohistochemistry. Both TuJ1 and H3P antibodies were generated in rabbits, but easily were distinguished based on characteristic staining patterns. E,H: Quantitative analysis of TuJ1 immunoreactivity in Ret+ cells shows that treatment with testosterone or MIS does not alter expression of the neuronal differentiation marker recognized by TuJ1 in the presence or absence of GDNF. F,I: Quantitative analysis of proliferation (H3P+ cells) shows that neither testosterone nor MIS influences proliferation of Ret+TuJ1+ cells in the presence or absence of GDNF. G,J: Quantitative analysis of proliferating Ret+TuJ1- cell demonstrates increased proliferation in response to testosterone, but not MIS in the absence of GDNF. There was no additive effect of testosterone plus GDNF under these conditions. Asterisks indicate P < n =" 3" bar =" 50">

Customer Data on New Alpha 2c

Alpha 2c staining of rat ventral spinal cord (dilution 1:100). para-picric fixative. Please note perinuclear labeling. This appears around most neurons in spinal cord.

Thank you to Dr. Patrick Carr, UND

GDF-3 and Cancer, Diabetes

We have just added a new GDF-3 to our BMPs/GDFs antibodies category.

Image: GDF Staining of mouse thymus. Tissue was stained with goat HRP-DAB (brown) and counterstained with Hematoxylin (blue

Description: The TGFp superfamily of ligands plays key functions in development and disease. In both human and mouse embryonic stem cells, a member of this family, GDF3, is specifically expressed in the pluripotent state. We show thatGDF3 is an inhibitor of its own subfamily, blocks classic BMP signaling in multiple contexts, interacts with BMP proteins and is expressed specifically in the node during gastrulation in a pattern consistent with BMP inhibition. Furthermore, we use gain- and reduction-of-function to show that in a species-specific manner, GDF3 regulates both of the two major characteristics of embryonic stem cells: the ability to maintain the undifferentiated state and the ability to differentiate into the full spectrum of cell.

GDF-3 is suspected to be expressed in certain forms of cancer. These could include Breast and Testicular Cancer (Link).

mGluR1 Alpha, Alpha 2c and TRPV1 Westerns

mGluR1 alpha (Catalog#: RA19065)

Species: Human, Mouse an Rat

Applications: ICC; IHC and WB

Immunogen: 1159-1171 SSVPSSPVSESVL GAEGGAGGADGQGAGP

VR1 (TRPV1) (Catalog#: GT15129)

Species: Rat

Applications: IHC and WB

KLH-coupled synthetic peptide corresponding to amino acid residues 4 - 21 of Vanilloid Receptor 1

2c Adrenergic Receptor (Catalog#: RA19064)

Species: Human and Rat

Applications: ICC; IHC and WB

Immunogen: 309-324