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WB: Wild type and PARK7 (DJ-1) KO animals
Data courtesy of Amanda Ashley and Dr. Marie Legare, Colorado State University.
Western conditions:
– 25mg protein from mouse brain lysate
– 1:2000 Park7 (DJ-1) antibody (Cat # RA19006)
– 12% gel
– Electrotransferred onto PVDF
– Blocked with 5% BSA in TBS-Tween
– Detected with ImmunStar HRP (BioRad)
– Dually probed for beta-actin (1:1000, Sigma)
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