Neuromics' Human Brian Microvascular Endothelial Cells in Action

Fc-saxatilin inhibits VEGF-induced permeability by regulating claudin-5 expression in human brain microvascular endothelial cells (HBMVECs)

Neuromics' cells were at the heart of this study.

Highlights: 

Fc-saxatilin prevents VEGF-induced permeability in human brain microvascular cells (HBMVECs). 

Fc-saxatilin inhibits VEGF-induced Src and Fak phosphorylation in HBMVECs. 

Fc-saxatilin blocks the downregulation of claudin-5 expression by VEGF in HBMVECs.

Image: HMBECS in Culture

Protocol: Human brain microvascular endothelial cells (HBMVECs) were purchased from Neuromics (HEC02; Minneapolis, MN, USA). HBMVECs were maintained in ENDO-growth medium (MED001; Neuromics). 

Here's a link to the full study: https://www.sciencedirect.com/science/article/pii/S0026286219301177#!

TRPV1 and Migraines

New Links to Insulin

Hungarian researchers used our guinea pig polyclonal TRPV1 receptor antibody (GP14100) to help answer why heightened insulin levels can lead to migraines.

Immunohistochemistry in rat trigeminal ganglion. (A) Trigeminal ganglion neurons in the ophthalmic division immunoreactive for insulin receptor (InsR), TRPV1 receptor and CGRP. (B) Coexpression of insulin receptor (InsR) with TRPV1 receptor and/or CGRP in trigeminal ganglion neurons. Colours representing insulin receptor-, TRPV1 receptor- and CGRP-immunoreactivities and their coexpressions indicated in B applies also to A. (C) A trigeminal ganglion neuron retrogradely labeled with True blue expresses both insulin receptor (InsR) and TRPV1 receptor

Conclusion-the present findings indicate that insulin may activate TRPV1 receptors in the trigeminovascular system. Modified TRPV1 receptor function induced by insulin may also increase the sensitivity of both neural and vascular TRPV1 receptor for its agonists. Our data may provide a pathophysiological basis for the increased incidence of migraine in patients with hyperinsulin levels.