No Doubt!
Sometimes we hear our human cells did not express a certain receptor in the hands of our customers. We guarantee our cell "walk and talk" as advertised. If you are ever in doubt. we will test for expression of any receptors or cytoplasmic proteins. We also do this as part of our quality checks.
Here're, for example, is a pub showing our Schwann Cells working. Note the receptors tested here. Seung Min Shin, Francie Moehring, Brandon Itson-Zoske, Fan Fan, Cheryl L. Stucky, Quinn H. Hogan, and Hongwei Yu. (2021). Piezo2 Mechanosensitive Ion Channel is Located to Sensory Neurons and Non-Neuronal Cells in Rat Peripheral Sensory Pathway: Implications in Pain. bioRxiv. doi: 10.1101/2021.01.20.427483
Figure. IHC delineation of Piezo2 (PZ2) axonal component and Schwann cell expression. Representative montage images on sciatic nerve cryosections show double immunostaining (IS) of Piezo2 (red) with a selection of neuronal markers (green), including Tubb3 (A), NF200 (B), IB4 (C), and CGRP (D). Representative montage images on sciatic nerve cryosections show double- IS of Piezo2 (red) with glia markers (green), including S100 (E), p75NTR (F), GFAP (G), and MBP (H). Representative montage images on human Schwann cells (I) and isolated Schwann cells
(J) from rat sciatic nerve show double-IS of Piezo2 (red) with S100 (green). Scale bars: 50 um for all.
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