Friday, November 13, 2009

NPY Y2R and IHC-Mouse Distal Colon

Lixin Wang, Guillaume Gourcerol, Pu-Qing Yuan, S. Vincent Wu, Mulugeta Million, Muriel Larauche, and Yvette Taché. Peripheral peptide YY inhibits propulsive colonic motor function through Y2 receptor in conscious mice. Am J Physiol Gastrointest Liver Physiol (November 5, 2009). doi:10.1152/ajpgi.00349.2009.
...Note: Excellent IHC staining of myenteric plexus and submucosal (mouse distal colon) tissue-Free floating submucosal and LMMP whole mounts of both proximal and distal colon from 3 naïve mice were treated in 10% normal goat serum each for 30 min, and followed by incubation with polyclonal rabbit anti- NPY Y2 Receptor diluted at 1:1,000 (Neuromics, Inc., Edina, MN)...

Related Antibodies to Consider:
NPY Y2 Receptor-C/N Terminus
NPY Y1 Receptor
All Neuropeptide and Neuropeptide Receptor Antibodies
Pain and Inflammation Research Antibodies
Diabetes and Obesity Research Antibodies

More Pubs Referencing Neuromics' mGluRs

Jakob S. Satz, Alisdair R. Philp, Huy Nguyen, Hajime Kusano, Jane Lee, Rolf Turk, Megan J. Riker, Jasmine Hernández, Robert M. Weiss, Michael G. Anderson, Robert F. Mullins, Steven A. Moore, Edwin M. Stone, and Kevin P. Campbell. Visual Impairment in the Absence of Dystroglycan. J. Neurosci., Oct 2009; 29: 13136 - 13146 ; doi:10.1523/JNEUROSCI.0474-09.2009.
....anti-mGluR6 (Neuromics)...

Lasani S. Wijetunge, Sally M. Till, Thomas H. Gillingwater, Cali A. Ingham, and Peter C. Kind. mGluR5 Regulates Glutamate-Dependent Development of the Mouse Somatosensory Cortex. The Journal of Neuroscience, December 3, 2008, 28(49):13028-13037; doi:10.1523/JNEUROSCI.2600-08.2008.
...Western blotting was performed as mentioned above and membranes were probed with antibodies against mGluR5 (1:4000, Neuromics)...
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Friday, November 06, 2009

gp130, IL-6 and Expression and Neuropathic Pain

We wanted to post yet another publication referencing use of one of our Pain and Inflammation Research Antibodies. Here the researchers used our Mouse Monoclonal gp130/CD130 for Immunohistochemistry.

Manfred Andratsch, Norbert Mair, Cristina E. Constantin, Nadja Scherbakov, Camilla Benetti, Serena Quarta, Christian Vogl, Claudia A. Sailer, Nurcan Üceyler, Johannes Brockhaus, Rudolf Martini, Claudia Sommer, Hanns Ulrich Zeilhofer, Werner Müller, Rohini Kuner, John B. Davis, Stefan Rose-John, and Michaela Kress. A Key Role for gp130 Expressed on Peripheral Sensory Nerves in Pathological Pain. J. Neurosci., Oct 2009; 29: 13473 - 13483 ; doi:10.1523/JNEUROSCI.1822-09.2009

The results suggest that gp130 expressed in sensory nerves not only mediates chronic inflammatory pain, but also contributes significantly to complex interactions between immune cells, tumor cells, and nerves in the context of cancer-evoked pain. Moreover, we identify IL-6 activating gp130, Gab1/Gab2, PI3K, and PKC- and regulating TRPV1 as a key mechanism linking cytokine release to sensitization of pain-sensing nerves.

Related Links
Immune Response
Opioid Neuropeptides
Opioid Receptors
Neurotransmissiom Research Antibodies
GPCRs, Ligand Gated Ion Channels, Biogenic Amines and more
Purinergic Receptors
TRPV (Vanilloid); TRPM; TRPA and TRPCs
i-Fect Transfection Kit
gene silencing of DOR, NaV1.8 tetrodotoxin-resistant sodium channel, NTS2 and more in-vitro and in vivo
Primary Neurons and Astrocytes
Primary human, rat and mouse neurons and astrocytes by Category